Dietary naringenin increases hepatic peroxisome proliferators-activated receptor α protein expression and decreases plasma triglyceride and adiposity in rats

Kae Won Cho, Yong Ook Kim, Juan Andrade, John R. Burgess, Young Cheul Kim

Research output: Contribution to journalArticle

Abstract

Background: Naringenin, a flavonoid present in grapefruit, has recently been shown to exert hypolipidemic and hypocholesterolemic effects, which has a particular importance for protecting against chronic diseases. However, the lipid-lowering potential of naringenin at the concentrations in the dietary range and its underlying mechanisms have yet to be fully elucidated. Aim: The aim of the present study was (1) to investigate the effects of dietary naringenin on plasma and hepatic triglyceride and cholesterol levels and on adipose deposition in rat and (2) to determine the contribution of hepatic peroxisome proliferators-activated receptor a (PPARα) expression to fatty acid oxidation. Methods: Male Long-Evans hooded rats were fed a diet supplemented with naringenin (0.003, 0.006, and 0.012%) for 6 weeks. We analyzed plasma and hepatic lipid contents and determined the protein expression of PPARα, carnitine-palmitoyl transferase 1L (CPT-1), and uncoupling protein 2 (UCP2), all of which are critical genes for fatty acid oxidation. Results: Naringenin supplementation caused a significant reduction in the amount of total triglyceride and cholesterol in plasma and liver. In addition, naringenin supplementation lowered adiposity and triglyceride contents in parametrial adipose tissue. Naringenin-fed animals showed a significant increase in PPARa protein expression in the liver. Furthermore, expression of CPT-1 and UCP2, both of which are known to be regulated by PPARa, was markedly enhanced by naringenin treatment. Conclusions: Our results indicate that the activation of PPARα transcription factor and upregulation of its fatty acid oxidation target genes by dietary naringenin may contribute to the hypolipidemic and anti-adiposity effects in vivo.

Original languageEnglish (US)
Pages (from-to)81-88
Number of pages8
JournalEuropean Journal of Nutrition
Volume50
Issue number2
DOIs
StatePublished - Mar 1 2011
Externally publishedYes

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Peroxisome Proliferator-Activated Receptors
Adiposity
Triglycerides
Liver
Proteins
Fatty Acids
Carnitine
Transferases
Cholesterol
Citrus paradisi
Lipids
naringenin
Long Evans Rats
Flavonoids
Genes
Adipose Tissue
Chronic Disease
Transcription Factors
Up-Regulation
Diet

Keywords

  • Anti-adiposity
  • CPT-1
  • Hypolipidemic effect
  • In vivo
  • Naringenin
  • PPAR-α
  • UCP2

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Dietary naringenin increases hepatic peroxisome proliferators-activated receptor α protein expression and decreases plasma triglyceride and adiposity in rats. / Cho, Kae Won; Kim, Yong Ook; Andrade, Juan; Burgess, John R.; Kim, Young Cheul.

In: European Journal of Nutrition, Vol. 50, No. 2, 01.03.2011, p. 81-88.

Research output: Contribution to journalArticle

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AU - Cho, Kae Won

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AU - Andrade, Juan

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