Zooming in on biological processes with fluorescence nanoscopy

Utsav Agrawal, Daniel T. Reilly, Charles M. Schroeder

Research output: Contribution to journalReview article

Abstract

Fluorescence nanoscopy enables the study of biological phenomena at nanometer scale spatial resolution. Recent biological studies using fluorescence nanoscopy have showcased the ability of these techniques to directly observe protein organization, subcellular molecular interactions, structural dynamics, electrical signaling, and diffusion of cytosolic proteins at unprecedented spatial resolution. Super-resolution imaging techniques critically rely on bright fluorescent probes such as organic dyes or fluorescent proteins. Recently, these methods have been extended to live cells and multicolor, three-dimensional imaging, thereby providing exquisite spatiotemporal resolutions of the order of 10-20. nm and 1-2. s for subcellular imaging. Further improvements in image processing algorithms, labeling techniques, correlative microscopy, and development of advanced fluorescent probes will be required to achieve true molecular-scale resolution using these techniques.

Original languageEnglish (US)
Pages (from-to)646-653
Number of pages8
JournalCurrent Opinion in Biotechnology
Volume24
Issue number4
DOIs
StatePublished - Aug 1 2013

Fingerprint

Biological Phenomena
Fluorescent Dyes
Fluorescence
Proteins
Imaging techniques
Three-Dimensional Imaging
Molecular interactions
Structural dynamics
Labeling
Microscopy
Microscopic examination
Image processing
Coloring Agents
Dyes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Zooming in on biological processes with fluorescence nanoscopy. / Agrawal, Utsav; Reilly, Daniel T.; Schroeder, Charles M.

In: Current Opinion in Biotechnology, Vol. 24, No. 4, 01.08.2013, p. 646-653.

Research output: Contribution to journalReview article

Agrawal, Utsav ; Reilly, Daniel T. ; Schroeder, Charles M. / Zooming in on biological processes with fluorescence nanoscopy. In: Current Opinion in Biotechnology. 2013 ; Vol. 24, No. 4. pp. 646-653.
@article{21ede7865ac04b849cde70b81fb402e2,
title = "Zooming in on biological processes with fluorescence nanoscopy",
abstract = "Fluorescence nanoscopy enables the study of biological phenomena at nanometer scale spatial resolution. Recent biological studies using fluorescence nanoscopy have showcased the ability of these techniques to directly observe protein organization, subcellular molecular interactions, structural dynamics, electrical signaling, and diffusion of cytosolic proteins at unprecedented spatial resolution. Super-resolution imaging techniques critically rely on bright fluorescent probes such as organic dyes or fluorescent proteins. Recently, these methods have been extended to live cells and multicolor, three-dimensional imaging, thereby providing exquisite spatiotemporal resolutions of the order of 10-20. nm and 1-2. s for subcellular imaging. Further improvements in image processing algorithms, labeling techniques, correlative microscopy, and development of advanced fluorescent probes will be required to achieve true molecular-scale resolution using these techniques.",
author = "Utsav Agrawal and Reilly, {Daniel T.} and Schroeder, {Charles M.}",
year = "2013",
month = "8",
day = "1",
doi = "10.1016/j.copbio.2013.02.016",
language = "English (US)",
volume = "24",
pages = "646--653",
journal = "Current Opinion in Biotechnology",
issn = "0958-1669",
publisher = "Elsevier Limited",
number = "4",

}

TY - JOUR

T1 - Zooming in on biological processes with fluorescence nanoscopy

AU - Agrawal, Utsav

AU - Reilly, Daniel T.

AU - Schroeder, Charles M.

PY - 2013/8/1

Y1 - 2013/8/1

N2 - Fluorescence nanoscopy enables the study of biological phenomena at nanometer scale spatial resolution. Recent biological studies using fluorescence nanoscopy have showcased the ability of these techniques to directly observe protein organization, subcellular molecular interactions, structural dynamics, electrical signaling, and diffusion of cytosolic proteins at unprecedented spatial resolution. Super-resolution imaging techniques critically rely on bright fluorescent probes such as organic dyes or fluorescent proteins. Recently, these methods have been extended to live cells and multicolor, three-dimensional imaging, thereby providing exquisite spatiotemporal resolutions of the order of 10-20. nm and 1-2. s for subcellular imaging. Further improvements in image processing algorithms, labeling techniques, correlative microscopy, and development of advanced fluorescent probes will be required to achieve true molecular-scale resolution using these techniques.

AB - Fluorescence nanoscopy enables the study of biological phenomena at nanometer scale spatial resolution. Recent biological studies using fluorescence nanoscopy have showcased the ability of these techniques to directly observe protein organization, subcellular molecular interactions, structural dynamics, electrical signaling, and diffusion of cytosolic proteins at unprecedented spatial resolution. Super-resolution imaging techniques critically rely on bright fluorescent probes such as organic dyes or fluorescent proteins. Recently, these methods have been extended to live cells and multicolor, three-dimensional imaging, thereby providing exquisite spatiotemporal resolutions of the order of 10-20. nm and 1-2. s for subcellular imaging. Further improvements in image processing algorithms, labeling techniques, correlative microscopy, and development of advanced fluorescent probes will be required to achieve true molecular-scale resolution using these techniques.

UR - http://www.scopus.com/inward/record.url?scp=84880943233&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84880943233&partnerID=8YFLogxK

U2 - 10.1016/j.copbio.2013.02.016

DO - 10.1016/j.copbio.2013.02.016

M3 - Review article

C2 - 23498844

AN - SCOPUS:84880943233

VL - 24

SP - 646

EP - 653

JO - Current Opinion in Biotechnology

JF - Current Opinion in Biotechnology

SN - 0958-1669

IS - 4

ER -