TY - JOUR
T1 - ZFN, TALEN, and CRISPR/Cas-based methods for genome engineering
AU - Gaj, Thomas
AU - Gersbach, Charles A.
AU - Barbas, Carlos F.
N1 - Funding Information:
The authors are supported by the National Institutes of Health (Pioneer Award DP1CA174426 (CB) and DP2OD008586 (CG) and National Science Foundation (CBET-1151035). T.G. was supported by National Institute of General Medicine Sciences fellowship (T32GM080209). We apologize to those investigators whose important contributions may have been omitted due to space constraints.
PY - 2013/7
Y1 - 2013/7
N2 - Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and TALENs enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error-prone nonhomologous end joining or homology-directed repair at specific genomic locations. Here, we review achievements made possible by site-specific nuclease technologies and discuss applications of these reagents for genetic analysis and manipulation. In addition, we highlight the therapeutic potential of ZFNs and TALENs and discuss future prospects for the field, including the emergence of clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases.
AB - Zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) comprise a powerful class of tools that are redefining the boundaries of biological research. These chimeric nucleases are composed of programmable, sequence-specific DNA-binding modules linked to a nonspecific DNA cleavage domain. ZFNs and TALENs enable a broad range of genetic modifications by inducing DNA double-strand breaks that stimulate error-prone nonhomologous end joining or homology-directed repair at specific genomic locations. Here, we review achievements made possible by site-specific nuclease technologies and discuss applications of these reagents for genetic analysis and manipulation. In addition, we highlight the therapeutic potential of ZFNs and TALENs and discuss future prospects for the field, including the emergence of clustered regulatory interspaced short palindromic repeat (CRISPR)/Cas-based RNA-guided DNA endonucleases.
KW - Clustered regulatory interspaced short palindromic repeat
KW - Genome engineering
KW - Transcription activator-like effector nuclease
KW - Zinc-finger nuclease
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U2 - 10.1016/j.tibtech.2013.04.004
DO - 10.1016/j.tibtech.2013.04.004
M3 - Review article
C2 - 23664777
AN - SCOPUS:84879264708
SN - 0167-7799
VL - 31
SP - 397
EP - 405
JO - Trends in Biotechnology
JF - Trends in Biotechnology
IS - 7
ER -