Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-κB p65ser311 signaling via IκB-α and GSK-3β reduced phosphorylation

Sirima Puangpraphant, Vermont P. Dia, Elvira Demejia, Guadalupe Garcia, Mark A. Berhow, Matthew A. Wallig

Research output: Contribution to journalArticle

Abstract

Yerba mate tea (YMT) has a chemopreventive role in a variety of inflammatory diseases. The objective was to determine the capability of YMT and mate saponins to prevent azoxymethane (AOM)-induced colonic inflammation in rats. YMT (2% dry leaves, w/v, as a source of drinking fluid) (n = 15) and mate saponins (0.01% in the diet, at a concentration present in one cup of YMT) (n = 15) were given ad libitum to rats 2 weeks prior to AOM-injection until the end of the study; while control rats (n = 15) received a basal diet and drinking water. After 8-weeks of study, total colonic mucosa was scraped (n = 3 rats/group) and the remaining colons (n =12 rats/group) were cut into three equal sections and aberrant crypt foci (ACF) were analyzed. YMT reduced ACF formation from 113 (control group) to 89 (P < 0.05). YMT and mate saponins reduced the expression of proinflammatory molecules COX-2 and iNOS with concomitant reduction in p-p65 (P < 0.05). Immunohistochemical analysis of the formalin-fixed middle colons showed that YMT and mate saponins reduced the expression of p-p65ser311 by 45.7% and 43.1%, respectively, in comparison to the control (P < 0.05). In addition, the expression of molecules upstream of NF-κB such as p-IκB-α and p-GSK-3βY216 was downregulated by YMT 24.7% and 24.4%, respectively (P < 0.05). Results suggest the mechanism involved in the chemopreventive effect of YMT and mate saponin consumption in AOM induced-colonic inflammation in rats is through inhibition of NF-κB.

Original languageEnglish (US)
Pages (from-to)430-440
Number of pages11
JournalBioFactors
Volume39
Issue number4
DOIs
StatePublished - Jan 1 2013

Fingerprint

Ilex paraguariensis
Azoxymethane
Glycogen Synthase Kinase 3
Phosphorylation
Saponins
Tea
Rats
Colon
Inflammation
Aberrant Crypt Foci
Nutrition
Rat control
Diet
Molecules
Drinking Water
Formaldehyde
Drinking
Mucous Membrane
Down-Regulation

Keywords

  • Colon cancer
  • Inflammation
  • Mate saponins
  • NF-κB signaling
  • Yerba mate

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Clinical Biochemistry

Cite this

Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-κB p65ser311 signaling via IκB-α and GSK-3β reduced phosphorylation. / Puangpraphant, Sirima; Dia, Vermont P.; Demejia, Elvira; Garcia, Guadalupe; Berhow, Mark A.; Wallig, Matthew A.

In: BioFactors, Vol. 39, No. 4, 01.01.2013, p. 430-440.

Research output: Contribution to journalArticle

Puangpraphant, Sirima ; Dia, Vermont P. ; Demejia, Elvira ; Garcia, Guadalupe ; Berhow, Mark A. ; Wallig, Matthew A. / Yerba mate tea and mate saponins prevented azoxymethane-induced inflammation of rat colon through suppression of NF-κB p65ser311 signaling via IκB-α and GSK-3β reduced phosphorylation. In: BioFactors. 2013 ; Vol. 39, No. 4. pp. 430-440.
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abstract = "Yerba mate tea (YMT) has a chemopreventive role in a variety of inflammatory diseases. The objective was to determine the capability of YMT and mate saponins to prevent azoxymethane (AOM)-induced colonic inflammation in rats. YMT (2{\%} dry leaves, w/v, as a source of drinking fluid) (n = 15) and mate saponins (0.01{\%} in the diet, at a concentration present in one cup of YMT) (n = 15) were given ad libitum to rats 2 weeks prior to AOM-injection until the end of the study; while control rats (n = 15) received a basal diet and drinking water. After 8-weeks of study, total colonic mucosa was scraped (n = 3 rats/group) and the remaining colons (n =12 rats/group) were cut into three equal sections and aberrant crypt foci (ACF) were analyzed. YMT reduced ACF formation from 113 (control group) to 89 (P < 0.05). YMT and mate saponins reduced the expression of proinflammatory molecules COX-2 and iNOS with concomitant reduction in p-p65 (P < 0.05). Immunohistochemical analysis of the formalin-fixed middle colons showed that YMT and mate saponins reduced the expression of p-p65ser311 by 45.7{\%} and 43.1{\%}, respectively, in comparison to the control (P < 0.05). In addition, the expression of molecules upstream of NF-κB such as p-IκB-α and p-GSK-3βY216 was downregulated by YMT 24.7{\%} and 24.4{\%}, respectively (P < 0.05). Results suggest the mechanism involved in the chemopreventive effect of YMT and mate saponin consumption in AOM induced-colonic inflammation in rats is through inhibition of NF-κB.",
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