Yeast metabolic engineering for carbon dioxide fixation and its application

Soo Rin Kim, Soo Jung Kim, Sun Ki Kim, Seung Oh Seo, Sujeong Park, Jamin Shin, Jeong Sun Kim, Bo Ram Park, Yong Su Jin, Pahn Shick Chang, Yong Cheol Park

Research output: Contribution to journalReview articlepeer-review


As numerous industrial bioprocesses rely on yeast fermentation, developing CO2-fixing yeast strains can be an attractive option toward sustainable industrial processes and carbon neutrality. Recent studies have shown that the expression of ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO) in yeasts, such as Saccharomyces cerevisiae and Kluyveromyces marxianus, enables mixotrophic CO2 fixation and production of biofuels. Also, the expression of a synthetic Calvin-Benson-Bassham (CBB) cycle including RuBisCO in Pichia pastoris enables autotrophic growth on CO2. This review highlights recent advances in metabolic engineering strategies to enable CO2 fixation in yeasts. Also, we discuss the potentials of other natural and synthetic metabolic pathways independent of RuBisCO for developing CO2-fixing yeast strains capable of producing value-added biochemicals.

Original languageEnglish (US)
Article number126349
JournalBioresource Technology
StatePublished - Feb 2022


  • Calvin-Benson-Bassham (CBB) cycle
  • Carbon dioxide fixation
  • Ribulose-1,5-bisphosphate carboxylase-oxygenase (RuBisCO)
  • Yeast metabolic engineering

ASJC Scopus subject areas

  • Bioengineering
  • Waste Management and Disposal
  • Environmental Engineering
  • Renewable Energy, Sustainability and the Environment


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