Yeast cell surface display of proteins and uses thereof

Michele Kieke; David M Kranz; Eric Boder; Karl Dane Wittrup

Yeast cell surface display of proteins and uses thereof

Michele Kieke (Inventor), David M Kranz (Inventor), Eric Boder (Inventor), Karl Dane Wittrup (Inventor)

Research output: Patent

Abstract

The present invention provides a genetic method of tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. As one embodiment, attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. As another embodiment, T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.

Original language	English (US)
U.S. patent number	9139637
Filing date	2/12/13
State	Published - Sep 22 2015

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title = "Yeast cell surface display of proteins and uses thereof",

abstract = "The present invention provides a genetic method of tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. As one embodiment, attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. As another embodiment, T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.",

author = "Michele Kieke and Kranz, {David M} and Eric Boder and Wittrup, {Karl Dane}",

note = "FEDERAL FUNDING LEGEND This invention was produced in part using funds obtained through a grant from the National Institutes of Health grant R01AI35990. Consequently, the federal government has certain rights in this invention.; 9139637",

year = "2015",

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day = "22",

language = "English (US)",

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TY - PAT

T1 - Yeast cell surface display of proteins and uses thereof

AU - Kieke, Michele

AU - Kranz, David M

AU - Boder, Eric

AU - Wittrup, Karl Dane

N1 - FEDERAL FUNDING LEGEND This invention was produced in part using funds obtained through a grant from the National Institutes of Health grant R01AI35990. Consequently, the federal government has certain rights in this invention.

PY - 2015/9/22

Y1 - 2015/9/22

N2 - The present invention provides a genetic method of tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. As one embodiment, attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. As another embodiment, T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.

AB - The present invention provides a genetic method of tethering polypeptides to the yeast cell wall in a form accessible for binding to macromolecules. Combining this method with fluorescence-activated cell sorting provides a means of selecting proteins with increased or decreased affinity for another molecule, altered specificity, or conditional binding. As one embodiment, attaching an scFv antibody fragment to the Aga2p agglutinin effectively mimics the cell surface display of antibodies by B cells in the immune system for affinity maturation in vivo. As another embodiment, T cell receptor mutants can be isolated by this method that are efficiently displayed on the yeast cell surface, providing a means of altering T cell receptor binding affinity and specificity by library screening.

M3 - Patent

M1 - 9139637

Y2 - 2013/02/12

ER -

Yeast cell surface display of proteins and uses thereof

Abstract

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