Yak (Bos Grunniens) stomach lysozyme: Molecular cloning, expression and its antibacterial activities

Mingfeng Jiang, Yan Chen, Yong Wang, Juan J. Loor, Yuhui Ye, Yongli Wen, Xiangdong Zi, Yingfan Cai, James K. Drackley

Research output: Contribution to journalArticle

Abstract

The cDNA coding for stomach lysozyme in yak was cloned. The cloned cDNA contains a 432bp open reading frame and encodes 143 amino acids (16.24KDa) with a signal peptide of 18 amino acids. Further analysis revealed that its amino acid sequence shares many common properties with cow milk lysozyme. Expression of this gene was also detected in mammary gland tissue by RT-PCR. Phylogenetic relationships among yak stomach lysozyme and 8 cow lysozymes indicated that the yak enzyme is more closely related to both cow milk lysozyme and the pseudogene NS4 than cow stomach lysozyme. Recombinant yak lysozyme purified by Ni2+-column showed a molecular weight of 33.78kDa and exhibited lytic activity against Staphylococcus aureus, providing evidence of its antibacterial activities.

Original languageEnglish (US)
Pages (from-to)25-35
Number of pages11
JournalAnimal Biotechnology
Volume21
Issue number1
DOIs
StatePublished - Jan 1 2010

Keywords

  • Expression
  • Phylogenetic tree
  • Stomach lysozyme
  • Yak

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Animal Science and Zoology

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