Yak (Bos Grunniens) stomach lysozyme: Molecular cloning, expression and its antibacterial activities

Mingfeng Jiang; Yan Chen; Yong Wang; Juan J. Loor; Yuhui Ye; Yongli Wen; Xiangdong Zi; Yingfan Cai; James K. Drackley

doi:10.1080/10495390903339899

Yak (Bos Grunniens) stomach lysozyme: Molecular cloning, expression and its antibacterial activities

Mingfeng Jiang, Yan Chen, Yong Wang, Juan J. Loor, Yuhui Ye, Yongli Wen, Xiangdong Zi, Yingfan Cai, James K. Drackley

Research output: Contribution to journal › Article › peer-review

Abstract

The cDNA coding for stomach lysozyme in yak was cloned. The cloned cDNA contains a 432bp open reading frame and encodes 143 amino acids (16.24KDa) with a signal peptide of 18 amino acids. Further analysis revealed that its amino acid sequence shares many common properties with cow milk lysozyme. Expression of this gene was also detected in mammary gland tissue by RT-PCR. Phylogenetic relationships among yak stomach lysozyme and 8 cow lysozymes indicated that the yak enzyme is more closely related to both cow milk lysozyme and the pseudogene NS4 than cow stomach lysozyme. Recombinant yak lysozyme purified by Ni2+-column showed a molecular weight of 33.78kDa and exhibited lytic activity against Staphylococcus aureus, providing evidence of its antibacterial activities.

Original language	English (US)
Pages (from-to)	25-35
Number of pages	11
Journal	Animal Biotechnology
Volume	21
Issue number	1
DOIs	https://doi.org/10.1080/10495390903339899
State	Published - Jan 2010

Keywords

Expression
Phylogenetic tree
Stomach lysozyme
Yak

ASJC Scopus subject areas

Biotechnology
Bioengineering
Animal Science and Zoology

Online availability

10.1080/10495390903339899

Library availability

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title = "Yak (Bos Grunniens) stomach lysozyme: Molecular cloning, expression and its antibacterial activities",

abstract = "The cDNA coding for stomach lysozyme in yak was cloned. The cloned cDNA contains a 432bp open reading frame and encodes 143 amino acids (16.24KDa) with a signal peptide of 18 amino acids. Further analysis revealed that its amino acid sequence shares many common properties with cow milk lysozyme. Expression of this gene was also detected in mammary gland tissue by RT-PCR. Phylogenetic relationships among yak stomach lysozyme and 8 cow lysozymes indicated that the yak enzyme is more closely related to both cow milk lysozyme and the pseudogene NS4 than cow stomach lysozyme. Recombinant yak lysozyme purified by Ni2+-column showed a molecular weight of 33.78kDa and exhibited lytic activity against Staphylococcus aureus, providing evidence of its antibacterial activities.",

keywords = "Expression, Phylogenetic tree, Stomach lysozyme, Yak",

author = "Mingfeng Jiang and Yan Chen and Yong Wang and Loor, {Juan J.} and Yuhui Ye and Yongli Wen and Xiangdong Zi and Yingfan Cai and Drackley, {James K.}",

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doi = "10.1080/10495390903339899",

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volume = "21",

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T2 - Molecular cloning, expression and its antibacterial activities

AU - Jiang, Mingfeng

AU - Chen, Yan

AU - Wang, Yong

AU - Loor, Juan J.

AU - Ye, Yuhui

AU - Wen, Yongli

AU - Zi, Xiangdong

AU - Cai, Yingfan

AU - Drackley, James K.

N1 - Funding Information: This work was supported by a grant from the Basic Research Program of Sichuan Province.

PY - 2010/1

Y1 - 2010/1

N2 - The cDNA coding for stomach lysozyme in yak was cloned. The cloned cDNA contains a 432bp open reading frame and encodes 143 amino acids (16.24KDa) with a signal peptide of 18 amino acids. Further analysis revealed that its amino acid sequence shares many common properties with cow milk lysozyme. Expression of this gene was also detected in mammary gland tissue by RT-PCR. Phylogenetic relationships among yak stomach lysozyme and 8 cow lysozymes indicated that the yak enzyme is more closely related to both cow milk lysozyme and the pseudogene NS4 than cow stomach lysozyme. Recombinant yak lysozyme purified by Ni2+-column showed a molecular weight of 33.78kDa and exhibited lytic activity against Staphylococcus aureus, providing evidence of its antibacterial activities.

AB - The cDNA coding for stomach lysozyme in yak was cloned. The cloned cDNA contains a 432bp open reading frame and encodes 143 amino acids (16.24KDa) with a signal peptide of 18 amino acids. Further analysis revealed that its amino acid sequence shares many common properties with cow milk lysozyme. Expression of this gene was also detected in mammary gland tissue by RT-PCR. Phylogenetic relationships among yak stomach lysozyme and 8 cow lysozymes indicated that the yak enzyme is more closely related to both cow milk lysozyme and the pseudogene NS4 than cow stomach lysozyme. Recombinant yak lysozyme purified by Ni2+-column showed a molecular weight of 33.78kDa and exhibited lytic activity against Staphylococcus aureus, providing evidence of its antibacterial activities.

KW - Expression

KW - Phylogenetic tree

KW - Stomach lysozyme

KW - Yak

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Yak (Bos Grunniens) stomach lysozyme: Molecular cloning, expression and its antibacterial activities

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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