@article{dfa4406cdf7c4ab5b7964d7dcf810ba8,
title = "Xanthene-Based Nitric Oxide-Responsive Nanosensor for Photoacoustic Imaging in the SWIR Window",
abstract = "Shortwave infrared (SWIR) dyes are characterized by their ability to absorb light from 900 to 1400 nm, which is ideal for deep tissue imaging owing to minimized light scattering and interference from endogenous pigments. An approach to access such molecules is to tune the photophysical properties of known near-infrared dyes. Herein, we report the development of a series of easily accessible (three steps) SWIR xanthene dyes based on a dibenzazepine donor conjugated to thiophene (SCR-1), thienothiophene (SCR-2), or bithiophene (SCR-3). We leverage the fact that SCR-1 undergoes a bathochromic shift when aggregated for in vivo studies by developing a ratiometric nanoparticle for NO (rNP-NO), which we employed to successfully visualize pathological levels of nitric oxide in a drug-induced liver injury model via deep tissue SWIR photoacoustic (PA) imaging. Our work demonstrates how easily this dye series can be utilized as a component in nanosensor designs for imaging studies.",
keywords = "D-A-D Xanthene Dyes, Drug-Induced Liver Injury, Nitric Oxide, Photoacoustic Imaging, SWIR",
author = "Rathnamalala, {Chathuranga S.L.} and Selena Hernandez and Lucero, {Melissa Y.} and Swartchick, {Chelsea B.} and {Kalam Shaik}, Abdul and Hammer, {Nathan I.} and East, {Amanda K.} and Gwaltney, {Steven R.} and Jefferson Chan and Scott, {Colleen N.}",
note = "This work was supported by the National Science Foundation for award OIA‐1757220 and under the Center for Chemical Innovation in Selective C−H Functionalization (CHE‐1700982), and the National Institutes of Health (R35GM133581 to J.C.). SH acknowledges the Alfred P. Sloan Foundation for support. MYL was supported by the Pines Graduate Fellowship. AKE acknowledges the Beckman Institute for Advanced Science for a graduate fellowship. J.C. thanks the Helen Corley Petit Scholar Program and the Camille and Henry Dreyfus Foundation. We also acknowledge the Molecular Imaging Laboratory at the Beckman Institute for use of the Nanozoomer, the veterinary histology, and diagnostic laboratories for performing H&E staining and liver function tests. Major funding for the 500 MHz Bruker CryoProbe was provided by the Roy J. Carver Charitable Trust (Muscatine, Iowa; Grant No. 15‐4521) to the School of Chemical Sciences NMR Lab. The Q‐Tof Ultima mass spectrometer was purchased in part with a grant from the National Science Foundation, Division of Biological Infrastructure (DBI‐0100085). The authors thank the Beckman Institute for Advance Sciences for partial support to upgrade the Quantamaster system for probe characterization. The authors also thank Mr. Zhenxiang Zhao for technical assistance for ex vivo experiments. This work was supported by the National Science Foundation for award OIA-1757220 and under the Center for Chemical Innovation in Selective C−H Functionalization (CHE-1700982), and the National Institutes of Health (R35GM133581 to J.C.). SH acknowledges the Alfred P. Sloan Foundation for support. MYL was supported by the Pines Graduate Fellowship. AKE acknowledges the Beckman Institute for Advanced Science for a graduate fellowship. J.C. thanks the Helen Corley Petit Scholar Program and the Camille and Henry Dreyfus Foundation. We also acknowledge the Molecular Imaging Laboratory at the Beckman Institute for use of the Nanozoomer, the veterinary histology, and diagnostic laboratories for performing H&E staining and liver function tests. Major funding for the 500 MHz Bruker CryoProbe was provided by the Roy J. Carver Charitable Trust (Muscatine, Iowa; Grant No. 15-4521) to the School of Chemical Sciences NMR Lab. The Q-Tof Ultima mass spectrometer was purchased in part with a grant from the National Science Foundation, Division of Biological Infrastructure (DBI-0100085). The authors thank the Beckman Institute for Advance Sciences for partial support to upgrade the Quantamaster system for probe characterization. The authors also thank Mr. Zhenxiang Zhao for technical assistance for ex vivo experiments.",
year = "2023",
month = mar,
day = "20",
doi = "10.1002/anie.202214855",
language = "English (US)",
volume = "62",
journal = "Angewandte Chemie - International Edition",
issn = "1433-7851",
publisher = "John Wiley & Sons, Ltd.",
number = "13",
}