X-ray reflectivity investigations of two-dimensional assemblies of C-cadherins: First steps in structural and functional studies

L. Martel, C. Johnson, S. Boutet, R. Al-Kurdi, O. Konovalov, I. Robinson, Deborah E Leckband, J. F. Legrand

Research output: Contribution to journalArticle

Abstract

Cadherins are transmembrane proteins involved in cell adhesion. They play a major role in recognition and adhesion between adjacent cells via calcium dependent interactions. Our studies aim at determining the laws of assembly of Cadherins and locating the adhesive interactions along the proteins, starting from low-resolution structures obtained by X-rays grazing incidence reflectivity. We have realized monolayers of the extracellular fragment of C-cadherin of the frog Xenopus, anchored to nickel chelating lipids at the water surface. From X-ray reflectivity measurements carried out at ESRF, we have elaborated profiles of the electron density of the proteins along their axis with 0.9 nm resolution. We have studied their adhesive behaviour with high and low calcium concentrations and we show an ordering of the protein above 1 mM of calcium. We present an attempt to locate the binding of a short fragment of Cadherin on the full-length protein. The complementarities of our results with those of biochemical studies should enable us to comprehend further cellular adhesion mechanisms.

Original languageEnglish (US)
JournalJournal De Physique. IV : JP
Volume12
Issue number6
DOIs
StatePublished - Jul 1 2002

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Cadherins
assemblies
proteins
Proteins
reflectance
X rays
calcium
Calcium
adhesion
x rays
adhesives
Adhesives
Adhesion
fragments
frogs
Cell adhesion
Chelation
Nickel
cells
surface water

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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X-ray reflectivity investigations of two-dimensional assemblies of C-cadherins : First steps in structural and functional studies. / Martel, L.; Johnson, C.; Boutet, S.; Al-Kurdi, R.; Konovalov, O.; Robinson, I.; Leckband, Deborah E; Legrand, J. F.

In: Journal De Physique. IV : JP, Vol. 12, No. 6, 01.07.2002.

Research output: Contribution to journalArticle

Martel, L. ; Johnson, C. ; Boutet, S. ; Al-Kurdi, R. ; Konovalov, O. ; Robinson, I. ; Leckband, Deborah E ; Legrand, J. F. / X-ray reflectivity investigations of two-dimensional assemblies of C-cadherins : First steps in structural and functional studies. In: Journal De Physique. IV : JP. 2002 ; Vol. 12, No. 6.
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