X-ray compatible microfluidic platforms for screening, crystallization and de novo structure determination of proteins

Sudipto Guha, Sarah L. Perry, Ashtamurthy S Pawate, Satish K Nair, Paul J A Kenis

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present here an X-ray compatible, array based microfluidic chip that functions as a complete crystallization platform. It is capable of screening multiple crystallization conditions using minimal amount of protein solution per trial and facilitates de novo structure determination by collecting and merging X-ray data from multiple crystalsgrown on-chip. Our approach bypasses issues associated with the need to grow large, high quality crystals, crystal damage during handling, cryocooling, and radiation exposure and enables in situ data collection at biologically relevant temperatures. To validate the method, we determined the structure of PhnA to 2.30Å resolution solely using data collected on-chip.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages876-878
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Fingerprint

Microfluidics
Screening
Crystallization
Proteins
X rays
Crystals
Merging
Radiation
Temperature

Keywords

  • In situ structure determination
  • Microfluidics
  • Protein crystallization
  • SAD

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Guha, S., Perry, S. L., Pawate, A. S., Nair, S. K., & Kenis, P. J. A. (2011). X-ray compatible microfluidic platforms for screening, crystallization and de novo structure determination of proteins. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 876-878). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

X-ray compatible microfluidic platforms for screening, crystallization and de novo structure determination of proteins. / Guha, Sudipto; Perry, Sarah L.; Pawate, Ashtamurthy S; Nair, Satish K; Kenis, Paul J A.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 876-878 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Guha, S, Perry, SL, Pawate, AS, Nair, SK & Kenis, PJA 2011, X-ray compatible microfluidic platforms for screening, crystallization and de novo structure determination of proteins. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, vol. 2, pp. 876-878, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.
Guha S, Perry SL, Pawate AS, Nair SK, Kenis PJA. X-ray compatible microfluidic platforms for screening, crystallization and de novo structure determination of proteins. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. p. 876-878. (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
Guha, Sudipto ; Perry, Sarah L. ; Pawate, Ashtamurthy S ; Nair, Satish K ; Kenis, Paul J A. / X-ray compatible microfluidic platforms for screening, crystallization and de novo structure determination of proteins. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. 2011. pp. 876-878 (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011).
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