TY - GEN
T1 - X-ray compatible microfluidic platforms for screening, crystallization and de novo structure determination of proteins
AU - Guha, Sudipto
AU - Perry, Sarah L.
AU - Pawate, Ashtamurthy S
AU - Nair, Satish K
AU - Kenis, Paul J A
PY - 2011
Y1 - 2011
N2 - We present here an X-ray compatible, array based microfluidic chip that functions as a complete crystallization platform. It is capable of screening multiple crystallization conditions using minimal amount of protein solution per trial and facilitates de novo structure determination by collecting and merging X-ray data from multiple crystalsgrown on-chip. Our approach bypasses issues associated with the need to grow large, high quality crystals, crystal damage during handling, cryocooling, and radiation exposure and enables in situ data collection at biologically relevant temperatures. To validate the method, we determined the structure of PhnA to 2.30Å resolution solely using data collected on-chip.
AB - We present here an X-ray compatible, array based microfluidic chip that functions as a complete crystallization platform. It is capable of screening multiple crystallization conditions using minimal amount of protein solution per trial and facilitates de novo structure determination by collecting and merging X-ray data from multiple crystalsgrown on-chip. Our approach bypasses issues associated with the need to grow large, high quality crystals, crystal damage during handling, cryocooling, and radiation exposure and enables in situ data collection at biologically relevant temperatures. To validate the method, we determined the structure of PhnA to 2.30Å resolution solely using data collected on-chip.
KW - In situ structure determination
KW - Microfluidics
KW - Protein crystallization
KW - SAD
UR - http://www.scopus.com/inward/record.url?scp=84883829586&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84883829586&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84883829586
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 876
EP - 878
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -