TY - JOUR
T1 - Wrinkled, wavelength-tunable graphene-based surface topographies for directing cell alignment and morphology
AU - Wang, Zhongying
AU - Tonderys, Daniel
AU - Leggett, Susan E.
AU - Williams, Evelyn Kendall
AU - Kiani, Mehrdad T.
AU - Spitz Steinberg, Ruben
AU - Qiu, Yang
AU - Wong, Ian Y.
AU - Hurt, Robert H.
N1 - Funding Information:
This work was supported by the U.S. National Science Foundation (Grants CBET-1132446 and INSPIRE Track 1 CBET-1344097 ), the Environmental Pathology Training Grant from the National Institutes of Health ( 5T32ES007272-23 , S.E.L.), the Karen T. Romer Undergraduate Teaching and Research Award (E.K.W.), the Vincent and Ruby DiMase Undergraduate Summer Fellowship (M.T.K.), seed funding from Jason and Donna McGraw Weiss ’89 (I.Y.W.) and startup funds from Brown University (I.Y.W.). We thank Profs. A.B. Kane and J.R. Morgan for their gifts of NIH-3T3 and normal human fibroblasts, respectively. We thank Prof. D. Hoffman-Kim for assistance with Oriana software. Technical discussions with Prof. K.S. Kim are gratefully acknowledged.
Publisher Copyright:
© 2015 Elsevier Ltd. All rights reserved.
PY - 2016/2/1
Y1 - 2016/2/1
N2 - Textured surfaces with periodic topographical features and long-range order are highly attractive for directing cell-material interactions. They mimic physiological environments more accurately than planar surfaces and can fundamentally alter cell alignment, shape, gene expression, as well as multicellular organization into hierarchical tissue architectures. Here we demonstrate for the first time that wrinkled graphene-based surfaces are suitable as textured cell attachment substrates, and that engineered wrinkling can dramatically alter cell alignment and morphology. The wrinkled surfaces are fabricated by graphene oxide wet deposition onto pre-stretched elastomers followed by relaxation and mild thermal treatment to stabilize the films in cell culture medium. Multilayer graphene oxide films form periodic, delaminated buckle textures whose wavelengths and amplitudes can be systematically tuned by variation in the wet deposition process. Human and murine fibroblasts attach to these textured films and remain viable, while developing pronounced alignment and elongation relative to those on planar graphene controls. Compared to lithographic patterning of nanogratings, this method has advantages in the simplicity and scalability of fabrication, as well as the opportunity to couple the use of topographic cues with the unique conductive, adsorptive, or barrier properties of graphene materials for functional biomedical devices.
AB - Textured surfaces with periodic topographical features and long-range order are highly attractive for directing cell-material interactions. They mimic physiological environments more accurately than planar surfaces and can fundamentally alter cell alignment, shape, gene expression, as well as multicellular organization into hierarchical tissue architectures. Here we demonstrate for the first time that wrinkled graphene-based surfaces are suitable as textured cell attachment substrates, and that engineered wrinkling can dramatically alter cell alignment and morphology. The wrinkled surfaces are fabricated by graphene oxide wet deposition onto pre-stretched elastomers followed by relaxation and mild thermal treatment to stabilize the films in cell culture medium. Multilayer graphene oxide films form periodic, delaminated buckle textures whose wavelengths and amplitudes can be systematically tuned by variation in the wet deposition process. Human and murine fibroblasts attach to these textured films and remain viable, while developing pronounced alignment and elongation relative to those on planar graphene controls. Compared to lithographic patterning of nanogratings, this method has advantages in the simplicity and scalability of fabrication, as well as the opportunity to couple the use of topographic cues with the unique conductive, adsorptive, or barrier properties of graphene materials for functional biomedical devices.
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U2 - 10.1016/j.carbon.2015.03.040
DO - 10.1016/j.carbon.2015.03.040
M3 - Article
AN - SCOPUS:84938670726
SN - 0008-6223
VL - 97
SP - 14
EP - 24
JO - Carbon
JF - Carbon
ER -