TY - JOUR
T1 - Wireless multi-lateral optofluidic microsystems for real-time programmable optogenetics and photopharmacology
AU - Wu, Yixin
AU - Wu, Mingzheng
AU - Vázquez-Guardado, Abraham
AU - Kim, Joohee
AU - Zhang, Xin
AU - Avila, Raudel
AU - Kim, Jin Tae
AU - Deng, Yujun
AU - Yu, Yongjoon
AU - Melzer, Sarah
AU - Bai, Yun
AU - Yoon, Hyoseo
AU - Meng, Lingzi
AU - Zhang, Yi
AU - Guo, Hexia
AU - Hong, Liu
AU - Kanatzidis, Evangelos E.
AU - Haney, Chad R.
AU - Waters, Emily A.
AU - Banks, Anthony R.
AU - Hu, Ziying
AU - Lie, Ferrona
AU - Chamorro, Leonardo P.
AU - Sabatini, Bernardo L.
AU - Huang, Yonggang
AU - Kozorovitskiy, Yevgenia
AU - Rogers, John A
N1 - This work made use of the NUFAB facility of Northwestern University’s NUANCE Center, which has received support from the SHyNE Resource (NSF ECCS-2025633), the IIN, and Northwestern’s MRSEC program (NSF DMR-1720139). Imaging work was performed at the Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. This work also made use of the MatCI Facility which receives support from the MRSEC Program (NSF DMR- 1720139) of the Materials Research Center at Northwestern University. Funding for the electronics and μ-fluidic systems was provided by the Querrey-Simpson Institute for Bioelectronics. Y.K. is supported by the NIH R01MH117111 and R01NS107539, One Mind Nick LeDeit Rising Star Award, Rita Allen Foundation Scholar Award, the Searle Scholar Award, and Beckman Young Investigator Award. M.W is supported as an affiliate fellow of the NIH T32 AG20506 and 2021 Christina Enroth-Cugell and David Cugell Fellow. Y.Z. is supported by the University of Connecticut start-up fund, NIH RF1NS118287, NIH R42MH116525, and NIH R61DA051489. R.A. acknowledges support from the National Science Foundation Graduate Research Fellowship (NSF grant number 1842165) and Ford Foundation Predoctoral Fellowship. NeuroLux acknowledges support from the NIH 5R42MH116525-03. S.M. is supported by the German Research Foundation (DFG) Postdoctoral Fellowship and The Ellen R. and Melvin J. Gordon Center Fellowship.
This work made use of the NUFAB facility of Northwestern University’s NUANCE Center, which has received support from the SHyNE Resource (NSF ECCS-2025633), the IIN, and Northwestern’s MRSEC program (NSF DMR-1720139). Imaging work was performed at the Northwestern University Center for Advanced Microscopy generously supported by NCI CCSG P30 CA060553 awarded to the Robert H Lurie Comprehensive Cancer Center. This work also made use of the MatCI Facility which receives support from the MRSEC Program (NSF DMR- 1720139) of the Materials Research Center at Northwestern University. Funding for the electronics and μ-fluidic systems was provided by the Querrey-Simpson Institute for Bioelectronics. Y.K. is supported by the NIH R01MH117111 and R01NS107539, One Mind Nick LeDeit Rising Star Award, Rita Allen Foundation Scholar Award, the Searle Scholar Award, and Beckman Young Investigator Award. M.W is supported as an affiliate fellow of the NIH T32 AG20506 and 2021 Christina Enroth-Cugell and David Cugell Fellow. Y.Z. is supported by the University of Connecticut start-up fund, NIH RF1NS118287, NIH R42MH116525, and NIH R61DA051489. R.A. acknowledges support from the National Science Foundation Graduate Research Fellowship (NSF grant number 1842165) and Ford Foundation Predoctoral Fellowship. NeuroLux acknowledges support from the NIH 5R42MH116525-03. S.M. is supported by the German Research Foundation (DFG) Postdoctoral Fellowship and The Ellen R. and Melvin J. Gordon Center Fellowship.
PY - 2022/12
Y1 - 2022/12
N2 - In vivo optogenetics and photopharmacology are two techniques for controlling neuronal activity that have immense potential in neuroscience research. Their applications in tether-free groups of animals have been limited in part due to tools availability. Here, we present a wireless, battery-free, programable multilateral optofluidic platform with user-selected modalities for optogenetics, pharmacology and photopharmacology. This system features mechanically compliant microfluidic and electronic interconnects, capabilities for dynamic control over the rates of drug delivery and real-time programmability, simultaneously for up to 256 separate devices in a single cage environment. Our behavioral experiments demonstrate control of motor behaviors in grouped mice through in vivo optogenetics with co-located gene delivery and controlled photolysis of caged glutamate. These optofluidic systems may expand the scope of wireless techniques to study neural processing in animal models.
AB - In vivo optogenetics and photopharmacology are two techniques for controlling neuronal activity that have immense potential in neuroscience research. Their applications in tether-free groups of animals have been limited in part due to tools availability. Here, we present a wireless, battery-free, programable multilateral optofluidic platform with user-selected modalities for optogenetics, pharmacology and photopharmacology. This system features mechanically compliant microfluidic and electronic interconnects, capabilities for dynamic control over the rates of drug delivery and real-time programmability, simultaneously for up to 256 separate devices in a single cage environment. Our behavioral experiments demonstrate control of motor behaviors in grouped mice through in vivo optogenetics with co-located gene delivery and controlled photolysis of caged glutamate. These optofluidic systems may expand the scope of wireless techniques to study neural processing in animal models.
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U2 - 10.1038/s41467-022-32947-0
DO - 10.1038/s41467-022-32947-0
M3 - Article
C2 - 36137999
AN - SCOPUS:85138352868
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 5571
ER -