Winter flounder antifreeze proteins: A multigene family

B. Gourlie, Y. Lin, J. Price, A. L. DeVries, D. Powers, R. C. Huang

Research output: Contribution to journalArticlepeer-review

Abstract

The nucleotide sequence of a cDNA clone of winter flounder antifreeze protein was determined by the dideoxynucleotide method. The sequence would predict a protein of 91 amino acids composed of a prepropeptide of 38 amino acids and a mature protein of 53 amino acids, which includes four complete 11-amino acid repeats. This predicted sequence corresponds to an antifreeze protein of intermediate size which is one 11-amino acid repeat longer than the smallest anti-freeze proteins found in the serum of winter flounder during the cold season. Southern blot hybridization analysis of winter flounder genomic DNA with radioactive cDNA probes reveals a multigene family of potential antifreeze protein genes. This conclusion is supported by amino acid sequence analysis of several serum antifreeze proteins.

Original languageEnglish (US)
Pages (from-to)14960-14965
Number of pages6
JournalJournal of Biological Chemistry
Volume259
Issue number23
StatePublished - Dec 1 1984
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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