Whole-Genome Sequencing of Porcine Epidemic Diarrhea Virus by Illumina MiSeq Platform

Leyi Wang, Tod Stuber, Patrick Camp, Suelee Robbe-Austerman, Yan Zhang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Porcine epidemic diarrhea virus (PEDV) belongs to the genus Alphacoronavirus of the family Coronaviridae. PEDV was identified as an emerging pathogen in US pig populations in 2013. Since then, this virus has been detected in at least 31 states in the USA and has caused significant economic loss to the swine industry. Active surveillance and characterization of PEDV are essential for monitoring the virus. Obtaining comprehensive information about the PEDV genome can improve our understanding of the evolution of PEDV viruses, and the emergence of new strains, and can enhance vaccine designs. In this chapter, both a targeted amplification method and a random-priming method are described to amplify the complete genome of PEDV for sequencing using the MiSeq platform. Overall, this protocol provides a useful two-pronged approach to complete whole-genome sequences of PEDV depending on the amount of virus in the clinical samples.
Original languageEnglish (US)
Title of host publicationAnimal Coronaviruses
EditorsLeyi Wang
PublisherHumana Press
Chapter18
Pages201-208
DOIs
StatePublished - Mar 16 2016
Externally publishedYes

Publication series

NameSpringer Protocols Handbooks
ISSN (Print)1949-2448
ISSN (Electronic)1949-2456

Keywords

  • Porcine epidemic diarrhea virus
  • Targeted amplification
  • SISPA
  • Whole-genome sequencing
  • MiSeq

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