Whole-Genome Sequencing of a Single Viral Species from a Highly Heterogeneous Sample

Hee Sun Han, Paul G. Cantalupo, Assaf Rotem, Shelley K. Cockrell, Martial Carbonnaux, James M. Pipas, David A. Weitz

Research output: Contribution to journalArticlepeer-review

Abstract

Metagenomic studies suggest that only a small fraction of the viruses that exist in nature have been identified and studied. Characterization of unknown viral genomes is hindered by the many genomes populating any virus sample. A new method is reported that integrates drop-based microfluidics and computational analysis to enable the purification of any single viral species from a complex mixed virus sample and the retrieval of complete genome sequences. By using this platform, the genome sequence of a 5243 bp dsDNA virus that was spiked into wastewater was retrieved with greater than 96 % sequence coverage and more than 99.8 % sequence identity. This method holds great potential for virus discovery since it allows enrichment and sequencing of previously undescribed viruses as well as known viruses.

Original languageEnglish (US)
Pages (from-to)13985-13988
Number of pages4
JournalAngewandte Chemie - International Edition
Volume54
Issue number47
DOIs
StatePublished - Nov 16 2015
Externally publishedYes

Keywords

  • genome sequencing
  • high-throughput screening
  • microemulsions
  • microfluidics
  • viruses

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)

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