Whole-genome resequencing identifies quantitative trait loci associated with mycorrhizal colonization of soybean

Michelle L. Pawlowski, Tri D. Vuong, Babu Valliyodan, Henry T. Nguyen, Glen L. Hartman

Research output: Contribution to journalArticle

Abstract

Key message: A whole-genome resequencing-derived SNP dataset identified six quantitative trait loci (QTL) significantly associated with colonization of soybean by an arbuscular mycorrhizal fungus (Rhizophagus intraradices). Candidate genes identified in these QTL regions include homologs to known nodulin protein families and other symbiosis-specific genes. Abstract: Arbuscular mycorrhizal fungi (AMF) form associations with over 80% of all terrestrial plant species and assist their host plants by increasing their nutrient uptake, drought tolerance, and resilience against pathogens and pests. Genotypic variation of crop plants to AMF colonization has been identified in crops, including soybean; however, the genetics controlling levels of AMF colonization in soybean are unknown. The overall goal of our study was to identify genomic regions associated with mycorrhizal colonization in soybean using genome-wide association analysis. A diverse panel of 350 exotic soybean genotypes inoculated with Rhizophagus intraradices were microscopically evaluated for root colonization using a modified gridline intersect method. Root colonization differed significantly (P < 0.001) among genotypes and ranged from 11 to 70%. A whole-genome resequencing-derived SNP dataset identified six quantitative trait loci (QTL) significantly associated with R. intraradices colonization that explained 24% of the phenotypic variance. Candidate genes identified in these QTL regions include homologs to known nodulin protein families and other symbiosis-specific genes. The results showed there was a significant genetic component to the level of colonization by R. intraradices in soybean. This information may be useful in the development of AMF-sensitive soybean cultivars to enhance nutrient uptake, drought tolerance, and disease resistance in the crop.

Original languageEnglish (US)
Pages (from-to)409-417
Number of pages9
JournalTheoretical and Applied Genetics
Volume133
Issue number2
DOIs
StatePublished - Feb 1 2020

Fingerprint

Quantitative Trait Loci
Soybeans
quantitative trait loci
Genome
soybeans
mycorrhizal fungi
genome
Fungi
nodulins
Symbiosis
Droughts
drought tolerance
nutrient uptake
symbiosis
Genes
Single Nucleotide Polymorphism
genes
crops
Genotype
Disease Resistance

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Whole-genome resequencing identifies quantitative trait loci associated with mycorrhizal colonization of soybean. / Pawlowski, Michelle L.; Vuong, Tri D.; Valliyodan, Babu; Nguyen, Henry T.; Hartman, Glen L.

In: Theoretical and Applied Genetics, Vol. 133, No. 2, 01.02.2020, p. 409-417.

Research output: Contribution to journalArticle

Pawlowski, Michelle L. ; Vuong, Tri D. ; Valliyodan, Babu ; Nguyen, Henry T. ; Hartman, Glen L. / Whole-genome resequencing identifies quantitative trait loci associated with mycorrhizal colonization of soybean. In: Theoretical and Applied Genetics. 2020 ; Vol. 133, No. 2. pp. 409-417.
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