Voluntary wheel running does not affect lipopolysaccharide-induced depressive-like behavior in young adult and aged mice

Stephen A. Martin, Robert Dantzer, Keith W. Kelley, Jeffrey A. Woods

Research output: Contribution to journalArticle

Abstract

Objective(s): Peripheral stimulation of the innate immune system with lipopolysaccharide (LPS) causes prolonged depressive-like behavior in aged mice that is dependent on indoleamine 2,3 dioxygenase (IDO) activation. Regular moderate-intensity exercise training has been shown to exert neuroprotective effects that might reduce depressive-like behavior in aged mice. The purpose of this study was to test the hypothesis that voluntary wheel running (VWR) would attenuate LPS-induced depressive-like behavior and brain IDO gene expression in 4- and 22-month-old C57BL/6J mice. Methods: Mice were housed with a running wheel (VWR) or no wheel (standard) for 30 (young adult mice) or 70 days (aged mice), after which they were intraperitoneally injected with LPS (young adult mice: 0.83 mg/kg; aged mice: 0.33 mg/kg). Results: Young adult VWR mice ran on average 6.9 km/day, while aged VWR mice ran on average 3.4 km/day. Both young adult and aged VWR mice increased their forced exercise tolerance compared to their respective standard control groups. VWR had no effect on LPS-induced anorexia, weight loss, increased immobility in the tail suspension test and decreased sucrose preference in either young adult or aged mice. Four (young adult mice) and 24 h (aged mice) after injection of LPS, mRNA transcripts for TNF- IL-1β, IL-6, and IDO were upregulated in the whole brain independently of VWR. Conclusion: Prolonged physical exercise has no effect on the neuroinflammatory response to LPS and its behavioral consequences in young adult and aged mice.

Original languageEnglish (US)
Pages (from-to)52-63
Number of pages12
JournalNeuroImmunoModulation
Volume21
Issue number1
DOIs
StatePublished - Dec 1 2013

Fingerprint

Running
Lipopolysaccharides
Young Adult
Indoleamine-Pyrrole 2,3,-Dioxygenase
Exercise
Hindlimb Suspension
Exercise Tolerance
Brain
Anorexia
Neuroprotective Agents
Interleukin-1
Inbred C57BL Mouse
Sucrose
Weight Loss
Immune System
Interleukin-6

Keywords

  • Aging
  • Depressive-like behavior
  • Proinflammatory cytokines
  • Sickness behavior
  • Voluntary wheel running

ASJC Scopus subject areas

  • Immunology
  • Endocrinology
  • Neurology
  • Endocrine and Autonomic Systems

Cite this

Voluntary wheel running does not affect lipopolysaccharide-induced depressive-like behavior in young adult and aged mice. / Martin, Stephen A.; Dantzer, Robert; Kelley, Keith W.; Woods, Jeffrey A.

In: NeuroImmunoModulation, Vol. 21, No. 1, 01.12.2013, p. 52-63.

Research output: Contribution to journalArticle

@article{1ccfc1766f4d45308983ef9442485298,
title = "Voluntary wheel running does not affect lipopolysaccharide-induced depressive-like behavior in young adult and aged mice",
abstract = "Objective(s): Peripheral stimulation of the innate immune system with lipopolysaccharide (LPS) causes prolonged depressive-like behavior in aged mice that is dependent on indoleamine 2,3 dioxygenase (IDO) activation. Regular moderate-intensity exercise training has been shown to exert neuroprotective effects that might reduce depressive-like behavior in aged mice. The purpose of this study was to test the hypothesis that voluntary wheel running (VWR) would attenuate LPS-induced depressive-like behavior and brain IDO gene expression in 4- and 22-month-old C57BL/6J mice. Methods: Mice were housed with a running wheel (VWR) or no wheel (standard) for 30 (young adult mice) or 70 days (aged mice), after which they were intraperitoneally injected with LPS (young adult mice: 0.83 mg/kg; aged mice: 0.33 mg/kg). Results: Young adult VWR mice ran on average 6.9 km/day, while aged VWR mice ran on average 3.4 km/day. Both young adult and aged VWR mice increased their forced exercise tolerance compared to their respective standard control groups. VWR had no effect on LPS-induced anorexia, weight loss, increased immobility in the tail suspension test and decreased sucrose preference in either young adult or aged mice. Four (young adult mice) and 24 h (aged mice) after injection of LPS, mRNA transcripts for TNF- IL-1β, IL-6, and IDO were upregulated in the whole brain independently of VWR. Conclusion: Prolonged physical exercise has no effect on the neuroinflammatory response to LPS and its behavioral consequences in young adult and aged mice.",
keywords = "Aging, Depressive-like behavior, Proinflammatory cytokines, Sickness behavior, Voluntary wheel running",
author = "Martin, {Stephen A.} and Robert Dantzer and Kelley, {Keith W.} and Woods, {Jeffrey A.}",
year = "2013",
month = "12",
day = "1",
doi = "10.1159/000356144",
language = "English (US)",
volume = "21",
pages = "52--63",
journal = "NeuroImmunoModulation",
issn = "1021-7401",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Voluntary wheel running does not affect lipopolysaccharide-induced depressive-like behavior in young adult and aged mice

AU - Martin, Stephen A.

AU - Dantzer, Robert

AU - Kelley, Keith W.

AU - Woods, Jeffrey A.

PY - 2013/12/1

Y1 - 2013/12/1

N2 - Objective(s): Peripheral stimulation of the innate immune system with lipopolysaccharide (LPS) causes prolonged depressive-like behavior in aged mice that is dependent on indoleamine 2,3 dioxygenase (IDO) activation. Regular moderate-intensity exercise training has been shown to exert neuroprotective effects that might reduce depressive-like behavior in aged mice. The purpose of this study was to test the hypothesis that voluntary wheel running (VWR) would attenuate LPS-induced depressive-like behavior and brain IDO gene expression in 4- and 22-month-old C57BL/6J mice. Methods: Mice were housed with a running wheel (VWR) or no wheel (standard) for 30 (young adult mice) or 70 days (aged mice), after which they were intraperitoneally injected with LPS (young adult mice: 0.83 mg/kg; aged mice: 0.33 mg/kg). Results: Young adult VWR mice ran on average 6.9 km/day, while aged VWR mice ran on average 3.4 km/day. Both young adult and aged VWR mice increased their forced exercise tolerance compared to their respective standard control groups. VWR had no effect on LPS-induced anorexia, weight loss, increased immobility in the tail suspension test and decreased sucrose preference in either young adult or aged mice. Four (young adult mice) and 24 h (aged mice) after injection of LPS, mRNA transcripts for TNF- IL-1β, IL-6, and IDO were upregulated in the whole brain independently of VWR. Conclusion: Prolonged physical exercise has no effect on the neuroinflammatory response to LPS and its behavioral consequences in young adult and aged mice.

AB - Objective(s): Peripheral stimulation of the innate immune system with lipopolysaccharide (LPS) causes prolonged depressive-like behavior in aged mice that is dependent on indoleamine 2,3 dioxygenase (IDO) activation. Regular moderate-intensity exercise training has been shown to exert neuroprotective effects that might reduce depressive-like behavior in aged mice. The purpose of this study was to test the hypothesis that voluntary wheel running (VWR) would attenuate LPS-induced depressive-like behavior and brain IDO gene expression in 4- and 22-month-old C57BL/6J mice. Methods: Mice were housed with a running wheel (VWR) or no wheel (standard) for 30 (young adult mice) or 70 days (aged mice), after which they were intraperitoneally injected with LPS (young adult mice: 0.83 mg/kg; aged mice: 0.33 mg/kg). Results: Young adult VWR mice ran on average 6.9 km/day, while aged VWR mice ran on average 3.4 km/day. Both young adult and aged VWR mice increased their forced exercise tolerance compared to their respective standard control groups. VWR had no effect on LPS-induced anorexia, weight loss, increased immobility in the tail suspension test and decreased sucrose preference in either young adult or aged mice. Four (young adult mice) and 24 h (aged mice) after injection of LPS, mRNA transcripts for TNF- IL-1β, IL-6, and IDO were upregulated in the whole brain independently of VWR. Conclusion: Prolonged physical exercise has no effect on the neuroinflammatory response to LPS and its behavioral consequences in young adult and aged mice.

KW - Aging

KW - Depressive-like behavior

KW - Proinflammatory cytokines

KW - Sickness behavior

KW - Voluntary wheel running

UR - http://www.scopus.com/inward/record.url?scp=84890469387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890469387&partnerID=8YFLogxK

U2 - 10.1159/000356144

DO - 10.1159/000356144

M3 - Article

VL - 21

SP - 52

EP - 63

JO - NeuroImmunoModulation

JF - NeuroImmunoModulation

SN - 1021-7401

IS - 1

ER -