TY - JOUR
T1 - Vitamin E imaging and localization in the neuronal membrane
AU - Monroe, Eric B.
AU - Jurchen, John C.
AU - Lee, Jinju
AU - Rubakhin, Stanislav S.
AU - Sweedler, Jonathan V.
PY - 2005/9/7
Y1 - 2005/9/7
N2 - Vitamin E (α-tocopherol) has been implicated in several cellular processes including signaling, transport, lipid membrane curvature, and several neurodegenerative disorders. Vitamin E imaging has been hindered by the inaccessibility of the molecule to traditional immunohistochemical methods. Using time-of-flight secondary ion mass spectrometry (ToF-SIMS), the distribution of major constituents in the cellular membrane of isolated neurons was investigated. There is a significant increase in the vitamin E signal at the soma-neurite junction compared to the cell as a whole (165 ± 11% of that found across the cell, p = 0.004, n = 12). The observed membrane distribution suggests an important new role for vitamin E in neuronal function.
AB - Vitamin E (α-tocopherol) has been implicated in several cellular processes including signaling, transport, lipid membrane curvature, and several neurodegenerative disorders. Vitamin E imaging has been hindered by the inaccessibility of the molecule to traditional immunohistochemical methods. Using time-of-flight secondary ion mass spectrometry (ToF-SIMS), the distribution of major constituents in the cellular membrane of isolated neurons was investigated. There is a significant increase in the vitamin E signal at the soma-neurite junction compared to the cell as a whole (165 ± 11% of that found across the cell, p = 0.004, n = 12). The observed membrane distribution suggests an important new role for vitamin E in neuronal function.
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U2 - 10.1021/ja051223y
DO - 10.1021/ja051223y
M3 - Article
C2 - 16131155
AN - SCOPUS:24644484701
SN - 0002-7863
VL - 127
SP - 12152
EP - 12153
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 35
ER -