Vitamin D is a regulator of endothelial nitric oxide synthase and arterial stiffness in mice

Olena Andrukhova, Svetlana Slavic, Ute Zeitz, Sabine C. Riesen, Monika S. Heppelmann, Tamas D. Ambrisko, Mato Markovic, Wolfgang M. Kuebler, Reinhold G. Erben

Research output: Contribution to journalArticle

Abstract

The vitamin D hormone 1α,25-dihydroxyvitamin D3 [1,25(OH)2D3] is essential for the preservation of serum calcium and phosphate levels but may also be important for the regulation of cardiovascular function. Epidemiological data in humans have shown that vitamin D insufficiency is associated with hypertension, left ventricular hypertrophy, increased arterial stiffness, and endothelial dysfunction in normal subjects and in patients with chronic kidney disease and type 2 diabetes. However, the pathophysiological mechanisms underlying these associations remain largely unexplained. In this study, we aimed to decipher the mechanisms by which 1,25(OH)2D3 may regulate systemic vascular tone and cardiac function, using mice carrying a mutant, functionally inactive vitamin D receptor (VDR). To normalize calcium homeostasis in VDR mutant mice, we fed the mice lifelong with the so-called rescue diet enriched with calcium, phosphate, and lactose. Here, we report that VDR mutant mice are characterized by lower bioavailability of the vasodilator nitric oxide (NO) due to reduced expression of the key NO synthesizing enzyme, endothelial NO synthase, leading to endothelial dysfunction, increased arterial stiffness, increased aortic impedance, structural remodeling of the aorta, and impaired systolic and diastolic heart function at later ages, independent of changes in the renin-angiotensin system. We further demonstrate that 1,25(OH)2D3 is a direct transcriptional regulator of endothelial NO synthase. Our data demonstrate the importance of intact VDR signaling in the preservation of vascular function and may provide a mechanistic explanation for epidemiological data in humans showing that vitamin D insufficiency is associated with hypertension and endothelial dysfunction.

Original languageEnglish (US)
Pages (from-to)53-64
Number of pages12
JournalMolecular Endocrinology
Volume28
Issue number1
DOIs
StatePublished - Jan 2014

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

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    Andrukhova, O., Slavic, S., Zeitz, U., Riesen, S. C., Heppelmann, M. S., Ambrisko, T. D., Markovic, M., Kuebler, W. M., & Erben, R. G. (2014). Vitamin D is a regulator of endothelial nitric oxide synthase and arterial stiffness in mice. Molecular Endocrinology, 28(1), 53-64. https://doi.org/10.1210/me.2013-1252