Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots

Chunmeng Shi; Ying Zhu; Zhihui Xie; Weiping Qian; Chia Ling Hsieh; Shuming Nie; Yongping Su; Haiyen E. Zhau; Leland W.K. Chung

doi:10.1016/j.urology.2009.01.023

Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots

Chunmeng Shi, Ying Zhu, Zhihui Xie, Weiping Qian, Chia Ling Hsieh, Shuming Nie, Yongping Su, Haiyen E. Zhau, Leland W.K. Chung

Research output: Contribution to journal › Article › peer-review

Abstract

Objectives: To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo. Methods: Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System. Results: As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors. Conclusions: Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.

Original language	English (US)
Pages (from-to)	446-451
Number of pages	6
Journal	Urology
Volume	74
Issue number	2
DOIs	https://doi.org/10.1016/j.urology.2009.01.023
State	Published - Aug 2009
Externally published	Yes

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Urology

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10.1016/j.urology.2009.01.023

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@article{b0a2d3dfc64c4fcca1c6d978c91e3282,

title = "Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots",

abstract = "Objectives: To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo. Methods: Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System. Results: As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors. Conclusions: Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.",

author = "Chunmeng Shi and Ying Zhu and Zhihui Xie and Weiping Qian and Hsieh, {Chia Ling} and Shuming Nie and Yongping Su and Zhau, {Haiyen E.} and Chung, {Leland W.K.}",

note = "Funding Information: This study was supported by grants from the National Institutes of Health (grant P01 CA098912 to L. W. K. Chung and grants R01 GM60562 and RO1 CA108468 to S. Nie), Department of Defense (grant 17-03-2-0033 to L. W. K. Chung), Georgia Cancer Coalition Distinguished Cancer Scholar Awards (L. W. K. Chung and S. Nie); and in part by grants NKBRP2005CB522605, SKLZZ200810, NFSC30400188, FANEDD200777, IRT0712 , and CSTC2008BB5023 from China (C. Shi and Y. Su). ",

year = "2009",

month = aug,

doi = "10.1016/j.urology.2009.01.023",

language = "English (US)",

volume = "74",

pages = "446--451",

journal = "Urology",

issn = "0090-4295",

publisher = "Elsevier Inc.",

number = "2",

}

TY - JOUR

T1 - Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots

AU - Shi, Chunmeng

AU - Zhu, Ying

AU - Xie, Zhihui

AU - Qian, Weiping

AU - Hsieh, Chia Ling

AU - Nie, Shuming

AU - Su, Yongping

AU - Zhau, Haiyen E.

AU - Chung, Leland W.K.

N1 - Funding Information: This study was supported by grants from the National Institutes of Health (grant P01 CA098912 to L. W. K. Chung and grants R01 GM60562 and RO1 CA108468 to S. Nie), Department of Defense (grant 17-03-2-0033 to L. W. K. Chung), Georgia Cancer Coalition Distinguished Cancer Scholar Awards (L. W. K. Chung and S. Nie); and in part by grants NKBRP2005CB522605, SKLZZ200810, NFSC30400188, FANEDD200777, IRT0712 , and CSTC2008BB5023 from China (C. Shi and Y. Su).

PY - 2009/8

Y1 - 2009/8

N2 - Objectives: To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo. Methods: Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System. Results: As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors. Conclusions: Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.

AB - Objectives: To visualize human prostate cancer cells in mouse bone with bioconjugated near-infrared quantum dot (QD) probes. Near-infrared fluorescent probes using QDs can visualize tumors in deep tissues in vivo. Methods: Human prostate cancer C4-2B xenografts grown in mouse tibia were detected by prostate-specific membrane antigen antibody conjugated with QDs emitting light at the near-infrared range of 800 nm (QD800). Images in culture and in vivo were acquired using the IVIS Imaging System. Results: As few as 5000 cells can be detected subcutaneously when tagged with QD800 conjugate and injected directly into mice. QD800 conjugate injected intravenously in mice harboring C4-2B tumors in tibia detected signals from a minimum of 500 000 cells. The maximal light emission was detected 30 minutes after intravenous injection of QD800 conjugate in mice with established C4-2B tumors. Conclusions: Bioconjugated near-infrared QD probes are highly sensitive molecular imaging tools for human prostate cancer micrometastases in mice.

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Visualizing Human Prostate Cancer Cells in Mouse Skeleton Using Bioconjugated Near-infrared Fluorescent Quantum Dots

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