TY - JOUR
T1 - Visualizing chromosome dynamics with GFP
AU - Belmont, Andrew S.
N1 - Funding Information:
This work was supported by grants from NIH to A.S.B. I thank Alan Grossman and Katherine Lemon for providing Fig. 2 , Susan M. Gasser for providing Fig. 5 and Andrew Murray for providing Fig. 6 for this paper. I thank Tudorita Tumbar for providing the right-hand panel of Fig. 4 . I also thank Julio Vazquez and John Sedat for sharing and allowing citation of unpublished data.
PY - 2001/6
Y1 - 2001/6
N2 - By allowing easy labeling of chromosomal and nuclear proteins and the tagging of specific chromosomal regions, the use of green-fluorescent protein (GFP) has provided new and special opportunities for directly observing chromosome dynamics in vivo. Here, we review recent applications of this methodology, focusing particularly on examples where new biology has been learned, or at least sighted. In particular, we focus on active bacterial chromosome segregation, yeast mitosis and centromere dynamics, and large-scale chromatin structure and dynamics within eukaryotic interphase nuclei.
AB - By allowing easy labeling of chromosomal and nuclear proteins and the tagging of specific chromosomal regions, the use of green-fluorescent protein (GFP) has provided new and special opportunities for directly observing chromosome dynamics in vivo. Here, we review recent applications of this methodology, focusing particularly on examples where new biology has been learned, or at least sighted. In particular, we focus on active bacterial chromosome segregation, yeast mitosis and centromere dynamics, and large-scale chromatin structure and dynamics within eukaryotic interphase nuclei.
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U2 - 10.1016/S0962-8924(01)02000-1
DO - 10.1016/S0962-8924(01)02000-1
M3 - Review article
C2 - 11356361
AN - SCOPUS:0035371920
SN - 0962-8924
VL - 11
SP - 250
EP - 257
JO - Trends in Cell Biology
JF - Trends in Cell Biology
IS - 6
ER -