Visualization of perforin/gasdermin/complement-formed pores in real cell membranes using atomic force microscopy

Yuying Liu, Tianzhen Zhang, Yabo Zhou, Jiping Li, Xiaoyu Liang, Nannan Zhou, Jiadi Lv, Jing Xie, Feiran Cheng, Yiliang Fang, Yunfeng Gao, Ning Wang, Bo Huang

Research output: Contribution to journalArticlepeer-review

Abstract

Different types of pores ubiquitously form in cell membranes, leading to various types of cell death that profoundly influence the fate of inflammation and the disease status. However, these pores have never truly been visualized to date. Atomic force microscopy (AFM), which is emerging as a powerful tool to analyze the mechanical properties of biomolecules and cells, is actually an excellent imaging platform that allows biological samples to be visualized by probing surface roughness at the level of atomic resolution. Here, membrane pore structures were clearly visualized using AFM. This visualization not only describes the aperture and depth of the pore complexes but also highlights differences among the pores formed by perforin and gasdermins in tumor cell membranes and by complement in immune cell membranes. Additionally, this type of visualization also reveals the dynamic process of pore formation, fusion, and repair.

Original languageEnglish (US)
Pages (from-to)611-620
Number of pages10
JournalCellular and Molecular Immunology
Volume16
Issue number6
DOIs
StatePublished - Jun 1 2019

Keywords

  • AFM
  • GSDMD/GSDME
  • complement
  • membrane pore formation
  • perforin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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