Viral transduction of male germline stem cells results in transgene transmission after germ cell transplantation in pigs

Wenxian Zeng, Lin Tang, Alla Bondareva, Ali Honaramooz, Valeria Tanco, Camila Dores, Susan Megee, Mark Modelski, Jose Rafael Rodriguez-Sosa, Melissa Paczkowski, Elena Silva, Matt Wheeler, Rebecca L. Krisher, Ina Dobrinski

Research output: Contribution to journalArticlepeer-review

Abstract

Genetic modification of germline stem cells (GSCs) is an alternative approach to generate large transgenic animals where transgenic GSCs are transplanted into a recipient testis to generate donor-derived transgenic sperm. The objective of the present study was to explore the application of viral vectors in delivering an enhanced green fluorescent protein (EGFP) transgene into GSCs for production of transgenic gametes through germ cell transplantation. Both adeno-associated virus (AAV)- and lentivirus (LV)-based vectors were effective in transducing pig GSCs, resulting in the production of transgenic sperm in recipient boars. Twenty-one boars treated with busulfan to deplete endogenous GSCs and nine nontreated boars received germ cell transplantation at 12 wk of age. Semen was collected from recipient boars from 5 to 7 mo posttrans- plantation when boars became sexually mature, and semen collection continued for as long as 5 yr for some boars. The percentage of ejaculates that were positive for the EGFP transgene ranged from 0% to 54.8% for recipients of AAV vector-transduced germ cells (n = 17) and from 0% to 25% for recipients of LV vector-transduced germ cells (n = 5). When semen from two AAV recipients was used for in vitro fertilization (IVF), 9.09% and 64.3% of embryos were trans- genic. Semen collected from two LV-vector recipients produced 7.7% and 26.3% transgenic IVF embryos. Here, we not only demonstrated AAV-mediated GSC transduction in another large animal model (pigs) but also showed, to our knowledge for the first time, that LV-mediated GSC transduction resulted in transgene transmission in pigs.

Original languageEnglish (US)
Article number27
JournalBiology of reproduction
Volume88
Issue number1
DOIs
StatePublished - Jan 2013

Keywords

  • Germ cell transplantation
  • Germline stem cells
  • Spermatogenesis
  • Spermatogonial stem cells
  • Transgenesis
  • Transgenic animal
  • Transplantation
  • Viral vectors

ASJC Scopus subject areas

  • Cell Biology

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