Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner

Quint Le Duc, Quanming Shi, Iris Blonk, Arnoud Sonnenberg, Ning Wang, Deborah Leckband, Johan De Rooij

Research output: Contribution to journalArticle

Abstract

Cell surface receptors integrate chemical and mechanical cues to regulate a wide range of biological processes. Integrin complexes are the mechanotransducers between the extracellular matrix and the actomyosin cytoskeleton. By analogy, cadherin complexes may function as mechanosensors at cell-cell junctions, but this capacity of cadherins has not been directly demonstrated. Furthermore, the molecular composition of the link between E-cadherin and actin, which is needed to sustain such a function, is unresolved. In this study, we describe nanomechanical measurements demonstrating that E-cadherin complexes are functional mechanosensors that transmit force between F-actin and E-cadherin. Imaging experiments reveal that intercellular forces coincide with vinculin accumulation at actin-anchored cadherin adhesions, and nanomechanical measurements show that vinculin potentiates the E-cadherin mechanosensory response. These investigations directly demonstrate the mechanosensory capacity of the E-cadherin complex and identify a novel function for vinculin at cell-cell junctions. These findings have implications for barrier function, morphogenesis, cell migration, and invasion and may extend to all soft tissues in which classical cadherins regulate cell-cell adhesion.

Original languageEnglish (US)
Pages (from-to)1107-1115
Number of pages9
JournalJournal of Cell Biology
Volume189
Issue number7
DOIs
StatePublished - Jun 28 2010

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Myosin Type II
Vinculin
Adherens Junctions
Cadherins
Actins
Intercellular Junctions
Biological Phenomena
Actomyosin
Cell Surface Receptors
Cytoskeleton
Morphogenesis
Cell Adhesion
Integrins
Cell Movement
Cues
Extracellular Matrix

ASJC Scopus subject areas

  • Cell Biology

Cite this

Vinculin potentiates E-cadherin mechanosensing and is recruited to actin-anchored sites within adherens junctions in a myosin II-dependent manner. / Le Duc, Quint; Shi, Quanming; Blonk, Iris; Sonnenberg, Arnoud; Wang, Ning; Leckband, Deborah; De Rooij, Johan.

In: Journal of Cell Biology, Vol. 189, No. 7, 28.06.2010, p. 1107-1115.

Research output: Contribution to journalArticle

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AU - Sonnenberg, Arnoud

AU - Wang, Ning

AU - Leckband, Deborah

AU - De Rooij, Johan

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