Video-rate large-scale imaging with multi-Z confocal microscopy

Amaury Badon, Seth Bensussen, Howard J. Gritton, Mehraj R. Awal, Christopher V. Gabel, Xue Han, Jerome Mertz

Research output: Contribution to journalArticlepeer-review


Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. To address this challenge, we report an augmented variant of confocal microscopy that uses a series of reflecting pinholes axially distributed in the detection space, such that each pinhole probes a different depth within the sample. We thus obtain simultaneous multiplane imaging without the need for axial scanning. Our microscope technique is versatile and configured here to provide two-color fluorescence imaging with a field of view larger than a millimeter at video rate. Its general applicability is demonstrated with neuronal imaging of both Caenorhabditis elegans and mouse brains in vivo.

Original languageEnglish (US)
Article number348815
Pages (from-to)389-395
Number of pages7
Issue number4
StatePublished - Apr 2019
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics


Dive into the research topics of 'Video-rate large-scale imaging with multi-Z confocal microscopy'. Together they form a unique fingerprint.

Cite this