Video-rate large-scale imaging with multi-Z confocal microscopy

Amaury Badon, Seth Bensussen, Howard J. Gritton, Mehraj R. Awal, Christopher V. Gabel, Xue Han, Jerome Mertz

Research output: Contribution to journalArticlepeer-review

Abstract

Fast, volumetric imaging over large scales has been a long-standing challenge in biological microscopy. To address this challenge, we report an augmented variant of confocal microscopy that uses a series of reflecting pinholes axially distributed in the detection space, such that each pinhole probes a different depth within the sample. We thus obtain simultaneous multiplane imaging without the need for axial scanning. Our microscope technique is versatile and configured here to provide two-color fluorescence imaging with a field of view larger than a millimeter at video rate. Its general applicability is demonstrated with neuronal imaging of both Caenorhabditis elegans and mouse brains in vivo.

Original languageEnglish (US)
Article number348815
Pages (from-to)389-395
Number of pages7
JournalOptica
Volume6
Issue number4
DOIs
StatePublished - Apr 2019
Externally publishedYes

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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