Viability staining of soybean suspension-cultured cells and a seedling stem cutting assay to evaluate phytotoxicity of Fusarium solani f. sp. glycines culture filtrates

S. Li, G. L. Hartman, J. M. Widholm

Research output: Contribution to journalArticlepeer-review

Abstract

The phytotoxicity of culture filtrates of Fusarium solani f. sp. glycines, the fungus causing sudden death syndrome (SDS) of soybean (Glycine max), was tested with a viability stain of soybean suspension-cultured cells and a stem cutting assay of soybean seedlings. Suspension-cultured cells from a SDS-susceptible soybean cultivar were exposed to cell-free culture filtrates of F. solani f. sp. glycines or other F. solani isolates for 2, 4, 6, and 8 days and then stained with 0.1% phenosafranin. The percentage of dead soybean suspension-cultured cells was greater (P<0.001) with tiltrates prepared from F. solani f. sp. glycines than from other F. solani isolates, and dead cells increased over time and with higher concentrations of culture filtrate. Cuttings of soybean seedlings with their stems immersed in culture filtrates of F. solani f. sp. glycines isolates developed SDS-like foliar symptoms, but not when immersed in filtrates of other isolates. There was a positive correlation (r=0.94, P<0.001) between soybean foliar symptom severity and percentage of stained soybean suspension-cultured cells. Both methods were used to determine the phytotoxicity of fungal culture filtrates.

Original languageEnglish (US)
Pages (from-to)375-380
Number of pages6
JournalPlant Cell Reports
Volume18
Issue number5
DOIs
StatePublished - Jan 1 1999

Keywords

  • Phytotoxicity
  • Soybean
  • Stem cutting assay
  • Suspension culture
  • Viability staining

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Fingerprint Dive into the research topics of 'Viability staining of soybean suspension-cultured cells and a seedling stem cutting assay to evaluate phytotoxicity of Fusarium solani f. sp. glycines culture filtrates'. Together they form a unique fingerprint.

Cite this