Viability and metal reduction of Shewanella oneidensis MR-1 under CO 2 stress: Implications for ecological effects of CO2 leakage from geologic CO2 sequestration

Bing Wu, Hongbo Shao, Zhipeng Wang, Yandi Hu, Yinjie J. Tang, Young Shin Jun

Research output: Contribution to journalArticle

Abstract

To study potential ecological impacts of CO2 leakage to shallow groundwater and soil/sediments from geologic CO2 sequestration (GCS) sites, this work investigated the viability and metal reduction of Shewanella oneidensis MR-1 under CO2 stress. While MR-1 could grow under high-pressure nitrogen gas (500 psi), the mix of 1% CO2 with N 2 at total pressures of 15 or 150 psi significantly suppressed the growth of MR-1, compared to the N2 control. When CO2 partial pressures were over 15 psi, the growth of MR-1 stopped. The reduced bacterial viability was consistent with the pH decrease and cellular membrane damage under high pressure CO2. After exposure to 150 psi CO 2 for 5 h, no viable cells survived, the cellular contents were released, and microscopy images confirmed significant cell structure deformation. However, after a relatively short exposure (25 min) to 150 psi CO2, MR-1 could fully recover their growth within 24 h after the stress was removed, and the reduction of MnO2 by MR-1 was observed right after the stress was removed. Furthermore, MR-1 survived better if the cells were aggregated rather than suspended, or if pH buffering minerals, such as calcite, were present. To predict the cell viability under different CO 2 pressures and exposure times, a two-parameter mathematical model was developed.

Original languageEnglish (US)
Pages (from-to)9213-9218
Number of pages6
JournalEnvironmental Science and Technology
Volume44
Issue number23
DOIs
StatePublished - Dec 1 2010
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

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