Vesicle budding induced by a pore-forming peptide

Yan Yu, Julie A. Vroman, Sung Chul Bae, Steve Granick

Research output: Contribution to journalArticlepeer-review

Abstract

We describe, in a system whose uniqueness is that the presence of pores allows the volume to vary as budding proceeds, how phase separation on the surface of spheres extrudes material in the process called "budding". The system is giant phospholipid vesicles (GUVs) containing phase-separated regions of DOPC (soft, liquid) and DPPC (stiff, gel), with cholesterol and without it. Budding is triggered by adding the cationic pore-forming peptide, melittin. Without cholesterol, fluorescence experiments show that melittin selectively binds to the liquid domains, inducing them to form mainly exocytotic monodisperse smaller vesicle buds of this same material, causing the parent GUV to shrink. The effect of cholesterol is to produce just a few large buds following domain coalescence, rather than numerous smaller monodisperse ones. Line tension is experimentally shown to be essential for budding in this multicomponent membrane.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalJournal of the American Chemical Society
Volume132
Issue number1
DOIs
StatePublished - Jan 13 2010

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Vesicle budding induced by a pore-forming peptide'. Together they form a unique fingerprint.

Cite this