Vertical Integration of Cell-Laden Hydrogels with Bioinspired Photonic Crystal Membranes

Yi Pei, Sayyed Hamed Shahoei, Yanfen Li, Peter J. Reece, Erik R. Nelson, J. Justin Gooding, Kristopher A. Kilian

Research output: Contribution to journalArticle

Abstract

The native architecture of tissue segregates populations of cells through nanostructured membranes of biopolymers (e.g., basement membranes) which serve the dual purpose of providing scaffolding for cells and filtration through size-exclusion. A multilayered approach to fabricate tissue scaffolds where populations of encapsulated cells are separated by porous silicon (PSi)-based photonic crystal membranes with nano- and microstructure that mimics the basement membranes is reported here. The PSi films are fabricated to display discreet photonic bandgaps such that remote optical interrogation provides a specific resonance for each film. Through careful control of surface chemistry, nanostructured PSi films are engineered to serve as optical biosensors and biomolecule release reservoirs, where the optical signature can report on these distinct functions remotely using a simple light source. The promise of this approach is demonstrated as a “smart” tissue scaffolding by monitoring matrix metalloprotease (MMP) activity from encapsulated multilayered co-cultures of mesenchymal stem cells and human microvascular endothelial cells, with concurrent attenuation of MMP activity through release of angiogenic-modulating compounds. The integration of optically registered biosensing and drug-release capabilities—within a multilayered hydrogel scaffold with multiple cell types—provides a new approach to tissue engineering where dynamic bioactivity can be monitored remotely in real-time.

Original languageEnglish (US)
Article number1801233
JournalAdvanced Materials Interfaces
Volume5
Issue number23
DOIs
StatePublished - Dec 7 2018

Fingerprint

Porous silicon
Photonic crystals
Hydrogels
Membranes
Biopolymers
Endothelial cells
Biomolecules
Bioactivity
Surface chemistry
Stem cells
Tissue engineering
Cell culture
Biosensors
Scaffolds
Photonics
Light sources
Nanostructures
Energy gap
Cells
Tissue

Keywords

  • biosensor
  • drug release
  • photonic crystals
  • porous silicon
  • tissue engineering

ASJC Scopus subject areas

  • Mechanics of Materials
  • Mechanical Engineering

Cite this

Vertical Integration of Cell-Laden Hydrogels with Bioinspired Photonic Crystal Membranes. / Pei, Yi; Shahoei, Sayyed Hamed; Li, Yanfen; Reece, Peter J.; Nelson, Erik R.; Gooding, J. Justin; Kilian, Kristopher A.

In: Advanced Materials Interfaces, Vol. 5, No. 23, 1801233, 07.12.2018.

Research output: Contribution to journalArticle

Pei, Yi ; Shahoei, Sayyed Hamed ; Li, Yanfen ; Reece, Peter J. ; Nelson, Erik R. ; Gooding, J. Justin ; Kilian, Kristopher A. / Vertical Integration of Cell-Laden Hydrogels with Bioinspired Photonic Crystal Membranes. In: Advanced Materials Interfaces. 2018 ; Vol. 5, No. 23.
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