Vascular lesions and s-thrombomodulin concentrations from auricular arteries of rabbits infused with microbubble contrast agent and exposed to pulsed ultrasound

James F. Zachary, James P. Blue, Rita J. Miller, William D. O'Brien

Research output: Contribution to journalArticlepeer-review

Abstract

Arterial injury resulting from the interaction of contrast agent (CA) with ultrasound (US) was studied in rabbit auricular arteries and assessed by histopathologic evaluation and s-thrombomodulin concentrations. Three sites on each artery were exposed (2.8 MHz, 5-min exposure duration, 10-Hz pulse repetition frequency, 1.4-μs pulse duration) using one of three in situ peak rarefactional pressures (0.85, 3.9 or 9.5 MPa). Saline, saline/CA, and saline/US infusion groups (n = 28) did not have histopathologic damage. The saline/CA/US infusion group (n = 10) at exposure conditions below the FDA mechanical index limit of 1.9 did not have histopathologic damage, whereas the saline/CA/US infusion group (n = 9) at exposure conditions above the FDA limit did have damage (5 of 9 arteries). Lesions were characteristic of acute coagulative necrosis. Mean s-thrombomodulin concentrations, a marker for endothelial cell injury, were highest in rabbits exposed to US at 0.85 and 3.9 MPa, suggesting that vascular injury may be physiological and not accompanied by irreversible cellular injury. (E-mail: zacharyj@uiuc.edu).

Original languageEnglish (US)
Pages (from-to)1781-1791
Number of pages11
JournalUltrasound in Medicine and Biology
Volume32
Issue number11
DOIs
StatePublished - Nov 2006

Keywords

  • Animal model
  • Auricular arteries
  • Contrast agent
  • Endothelial cells
  • Microbubbles
  • Pathology
  • Pulsed ultrasound
  • Rabbit
  • Thrombomodulin
  • Ultrasound

ASJC Scopus subject areas

  • Biophysics
  • Radiological and Ultrasound Technology
  • Acoustics and Ultrasonics

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