Variation of the respiration of protozoan cells with length of centrifuging

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Abstract

The respiration rate of the chlorotic, free-living flagellate protozoan, Euglena gracilis var. bacillaris, depends, at least in part, upon the amount of centrifuging the cells undergo before the measurements are made. Centrifugation is necessary to concentrate these and other cells to densities sufficient to measure oxygen uptake in a Warburg-type respirometer. The longer the cells are centrifuged prior to introduction into respiration vessels, the lower is their respiration rate. Cells centrifuged for a total of 30 min initially respire at a rate only 40% of the rate of cells centrifuged for only 1 min. The data indicate that both length of centrifuging and length of measurements are important in determining the oxygen uptake of cells.

Original languageEnglish (US)
Pages (from-to)242-247
Number of pages6
JournalAnalytical Biochemistry
Volume2
Issue number3
DOIs
StatePublished - Jun 1961
Externally publishedYes

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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