Variation in nuclear DNA content in Malus species and cultivated apples

Tatiana C. Tatum; Svetlana Stepanovic; D. P. Biradar; A. Lane Rayburn; Schuyler S. Korban

doi:10.1139/G05-033

Variation in nuclear DNA content in Malus species and cultivated apples

Tatiana C. Tatum, Svetlana Stepanovic, D. P. Biradar, A. Lane Rayburn, Schuyler S. Korban

Research output: Contribution to journal › Article › peer-review

Abstract

The nuclear DNA content for a group of 40 Malus species and hybrids has been estimated using flow cytometry. Estimates of nuclear DNA content for this germplasm collection range from 1.45 pg for Malus fusca (diploid) to 2.57 pg for Malus ioensis (triploid). Among diploids, the nuclear (2C) DNA ranges from 1.45 pg for M. fusca to 1.68 pg for Malus transitoria. Among triploids, the nuclear (3C) DNA content ranges from 2.37 pg / 3C for Malus sikkimensis to 2.57 pg / 3C for M. ioensis. Given the complexity of the apple genome and its suggested allopolyploid origin, the results obtained in this study confirm earlier reports that polyploids can easily withstand the loss of a certain amount of DNA, and that there is a slight tendency towards diminished haploid nuclear DNA content with increased polyploidy.

Original language	English (US)
Pages (from-to)	924-930
Number of pages	7
Journal	Genome
Volume	48
Issue number	5
DOIs	https://doi.org/10.1139/G05-033
State	Published - Oct 2005

Keywords

Apple
DNA content
Flow cytometry
Malus
Ploidy

ASJC Scopus subject areas

Biotechnology
Molecular Biology
Genetics

Online availability

10.1139/G05-033

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title = "Variation in nuclear DNA content in Malus species and cultivated apples",

abstract = "The nuclear DNA content for a group of 40 Malus species and hybrids has been estimated using flow cytometry. Estimates of nuclear DNA content for this germplasm collection range from 1.45 pg for Malus fusca (diploid) to 2.57 pg for Malus ioensis (triploid). Among diploids, the nuclear (2C) DNA ranges from 1.45 pg for M. fusca to 1.68 pg for Malus transitoria. Among triploids, the nuclear (3C) DNA content ranges from 2.37 pg / 3C for Malus sikkimensis to 2.57 pg / 3C for M. ioensis. Given the complexity of the apple genome and its suggested allopolyploid origin, the results obtained in this study confirm earlier reports that polyploids can easily withstand the loss of a certain amount of DNA, and that there is a slight tendency towards diminished haploid nuclear DNA content with increased polyploidy.",

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AU - Tatum, Tatiana C.

AU - Stepanovic, Svetlana

AU - Biradar, D. P.

AU - Rayburn, A. Lane

AU - Korban, Schuyler S.

PY - 2005/10

Y1 - 2005/10

N2 - The nuclear DNA content for a group of 40 Malus species and hybrids has been estimated using flow cytometry. Estimates of nuclear DNA content for this germplasm collection range from 1.45 pg for Malus fusca (diploid) to 2.57 pg for Malus ioensis (triploid). Among diploids, the nuclear (2C) DNA ranges from 1.45 pg for M. fusca to 1.68 pg for Malus transitoria. Among triploids, the nuclear (3C) DNA content ranges from 2.37 pg / 3C for Malus sikkimensis to 2.57 pg / 3C for M. ioensis. Given the complexity of the apple genome and its suggested allopolyploid origin, the results obtained in this study confirm earlier reports that polyploids can easily withstand the loss of a certain amount of DNA, and that there is a slight tendency towards diminished haploid nuclear DNA content with increased polyploidy.

AB - The nuclear DNA content for a group of 40 Malus species and hybrids has been estimated using flow cytometry. Estimates of nuclear DNA content for this germplasm collection range from 1.45 pg for Malus fusca (diploid) to 2.57 pg for Malus ioensis (triploid). Among diploids, the nuclear (2C) DNA ranges from 1.45 pg for M. fusca to 1.68 pg for Malus transitoria. Among triploids, the nuclear (3C) DNA content ranges from 2.37 pg / 3C for Malus sikkimensis to 2.57 pg / 3C for M. ioensis. Given the complexity of the apple genome and its suggested allopolyploid origin, the results obtained in this study confirm earlier reports that polyploids can easily withstand the loss of a certain amount of DNA, and that there is a slight tendency towards diminished haploid nuclear DNA content with increased polyploidy.

KW - Apple

KW - DNA content

KW - Flow cytometry

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KW - Ploidy

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Variation in nuclear DNA content in Malus species and cultivated apples

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