Abstract

Agarose hydrogels of varied elastic modulus can be patterned into 100-μm-wide channels with wall heights of 60 μm. After modifying the hydrogels with chloroacetic acid (acid gels), they are amenable to modification with amine-containing ligands using EDC-NHS chemistry. Using both rheometry and atomic force microscopy (AFM) nanoindentation measurements, the elastic modulus of unmodified hydrogels increases linearly from 3.6 ± 0.5 kPa to 45.2 ± 5.5 kPa for 0.5 to 2.0 wt/vol % hydrogel, respectively. The elastic modulus of acid gels is 2.2 ± 0.3 kPa to 16.2 ± 1.6 kPa for 0.5 to 2.0wt/vol %, respectively. No further changes were measured after further modifying the acid gels with fibronectin. Confocal images of rhodamine-modified acid gels show that the optimal filling viscosity of the agarose solutions is between 1 and 4 cP. This new method of patterning allows for the creation of substrates that take advantage of both micron-scale patterns and variably elastic hydrogels.

Original languageEnglish (US)
Pages (from-to)1483-1488
Number of pages6
JournalLangmuir
Volume23
Issue number3
DOIs
StatePublished - Jan 30 2007

Fingerprint

Hydrogels
Hydrogel
microchannels
Microchannels
Gels
acids
Acids
gels
Elastic moduli
modulus of elasticity
Sepharose
Chloroacetic acid
Rhodamines
Nanoindentation
Fibronectins
Amines
Atomic force microscopy
rhodamine
nanoindentation
Viscosity

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Variably elastic hydrogel patterned via capillary action in microchannels. / Dong, Rui; Jensen, Tor W.; Engberg, Kristin; Nuzzo, Ralph G.; Leckband, Deborah E.

In: Langmuir, Vol. 23, No. 3, 30.01.2007, p. 1483-1488.

Research output: Contribution to journalArticle

Dong, Rui ; Jensen, Tor W. ; Engberg, Kristin ; Nuzzo, Ralph G. ; Leckband, Deborah E. / Variably elastic hydrogel patterned via capillary action in microchannels. In: Langmuir. 2007 ; Vol. 23, No. 3. pp. 1483-1488.
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