Agarose hydrogels of varied elastic modulus can be patterned into 100-μm-wide channels with wall heights of 60 μm. After modifying the hydrogels with chloroacetic acid (acid gels), they are amenable to modification with amine-containing ligands using EDC-NHS chemistry. Using both rheometry and atomic force microscopy (AFM) nanoindentation measurements, the elastic modulus of unmodified hydrogels increases linearly from 3.6 ± 0.5 kPa to 45.2 ± 5.5 kPa for 0.5 to 2.0 wt/vol % hydrogel, respectively. The elastic modulus of acid gels is 2.2 ± 0.3 kPa to 16.2 ± 1.6 kPa for 0.5 to 2.0wt/vol %, respectively. No further changes were measured after further modifying the acid gels with fibronectin. Confocal images of rhodamine-modified acid gels show that the optimal filling viscosity of the agarose solutions is between 1 and 4 cP. This new method of patterning allows for the creation of substrates that take advantage of both micron-scale patterns and variably elastic hydrogels.

Original languageEnglish (US)
Pages (from-to)1483-1488
Number of pages6
Issue number3
StatePublished - Jan 30 2007

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry


Dive into the research topics of 'Variably elastic hydrogel patterned via capillary action in microchannels'. Together they form a unique fingerprint.

Cite this