Validation of a fiber-based confocal microscope for interventional image-guided procedures: Correlation with multispectral optical imaging

Daniel Herzka, Jade Quijano, Jianwu Xie, Sascha Krueger, Steffen Weiss, Benjamin Abrat, Anne Osdoit, Charlotte Cavé, Christopher Burnett, S. Narasimhan Danthi, King Li

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The concept of the biopsy is ubiquitous in current medical diagnosis of cancer and other diseases. The standard biopsy consists of removing a sample of tissue for evaluation and diagnosis, primarily to ascertain the presence of cancer cells by (histo)pathological analyses. However, the advent of new optical imaging modalities and targeted or "smart" agents, that have affinity for a select target, suggests the possibility of performing in vivo tissue characterization without the need for sample removal or the wait for histopathologic processing. Here we present work testing and validating a fiber-based confocal fluorescence microscopic imaging system intended for combination with a larger scale imaging modality (i.e. MRI or CT) to be used in image-guided in vivo tissue characterization. Fiber-based confocal fluorescence microscopic imaging experiments were performed (Cellvizio, Mauna Kea Technologies, Paris, France) in vivo in two mouse models including: 1) EGFP-expressing mouse melanoma model and 2) M21 mouse melanoma model. Both models are known to express integrin αvβ3, a cell-surface receptor protein. We also performed an experiment in ex vivo chicken muscle tissue labelled with a fluorescein isothiocyanate-lectin targeted compound. In the mouse models, contrast agents that targeted the integrin were injected and the contrast agent localization in tumor was verified by a whole-body multispectral imager. The fiber-based tool was sensitive enough to detect and image the tissue of interest in all different experiments, and was found appropriate for use in interventional catheter-based procedures.

Original languageEnglish (US)
Title of host publicationMedical Imaging 2006
Subtitle of host publicationPhysiology, Function, and Structure from Medical Images
DOIs
StatePublished - Jun 30 2006
Externally publishedYes
EventMedical Imaging 2006: Physiology, Function, and Structure from Medical Images - San Diego, CA, United States
Duration: Feb 12 2006Feb 14 2006

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6143 II
ISSN (Print)1605-7422

Other

OtherMedical Imaging 2006: Physiology, Function, and Structure from Medical Images
CountryUnited States
CitySan Diego, CA
Period2/12/062/14/06

Keywords

  • Image guided biopsy
  • Interventional optical imaging
  • Molecular imaging
  • Optical confocal fluorescence microscopy
  • Targeted optical imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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