Uterine development and fertility are dependent on gene dosage of the nuclear receptor coregulator REA

Sunghee Park, Sangyeon Yoon, Yuechao Zhao, Seong Eun Park, Lan Liao, Jianming Xu, John P. Lydon, Francesco J. DeMayo, Bert W. O'Malley, Milan K. Bagchi, Benita S. Katzenellenbogen

Research output: Contribution to journalArticle

Abstract

Although the effectiveness of nuclear hormone-receptor complexes is known to depend on coregulator partner proteins, relatively little is known about the roles of coregulators in uterine development and early stages of pregnancy and implantation. Because conventional genetic deletion of the coregulator, repressor of estrogen receptor activity (REA), was embryonic lethal, we here study REA conditional knockout mice generated by cre-loxP recombination, in which REA function was abrogated only in progesterone receptor-expressing tissues, to define the roles of REA in postembryonic stages and in a tissue-specific manner. We find that REA has gene dose-dependent activity impacting uterine development and fertility. Conditional homozygous mutant (REA d/d) mice developed to adulthood and showed normal ovarian function, but females were infertile with severely compromised uterine development and function characterized by cell cycle arrest, apoptosis, and altered adenogenesis (endometrial gland morphogenesis), resulting in failure of implantation and decidualization. By contrast, mice heterozygous for REA(REA f/d)had a very different phenotype, with estradiol treatment resulting in hyperstimulated, large uteri showing increased proliferation of luminal epithelial cells, and enhanced fluid imbibition associated with altered regulation of aquaporins. These REA f/d female mice showed a subfertility phenotype with reduced numbers and sizes of litters. These findings highlight that uterine development and regulation of estrogen receptor activities show a bimodal dependence on the gene dosage of REA. Optimal uterine development and functional activities require the normal gene dosage of REA, with partial or complete deletion resulting in hyperresponsiveness or underresponsiveness to hormone and subfertility or infertility, respectively.

Original languageEnglish (US)
Pages (from-to)3982-3994
Number of pages13
JournalEndocrinology
Volume153
Issue number8
DOIs
StatePublished - Aug 1 2012

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Gene Dosage
Cytoplasmic and Nuclear Receptors
Infertility
Fertility
Estrogen Receptors
Phenotype
Litter Size
Aquaporins
Progesterone Receptors
Cell Cycle Checkpoints
Morphogenesis
Knockout Mice
Genetic Recombination
Uterus
Estradiol
Epithelial Cells
Hormones
Apoptosis
Pregnancy
Genes

ASJC Scopus subject areas

  • Endocrinology

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Uterine development and fertility are dependent on gene dosage of the nuclear receptor coregulator REA. / Park, Sunghee; Yoon, Sangyeon; Zhao, Yuechao; Park, Seong Eun; Liao, Lan; Xu, Jianming; Lydon, John P.; DeMayo, Francesco J.; O'Malley, Bert W.; Bagchi, Milan K.; Katzenellenbogen, Benita S.

In: Endocrinology, Vol. 153, No. 8, 01.08.2012, p. 3982-3994.

Research output: Contribution to journalArticle

Park, S, Yoon, S, Zhao, Y, Park, SE, Liao, L, Xu, J, Lydon, JP, DeMayo, FJ, O'Malley, BW, Bagchi, MK & Katzenellenbogen, BS 2012, 'Uterine development and fertility are dependent on gene dosage of the nuclear receptor coregulator REA', Endocrinology, vol. 153, no. 8, pp. 3982-3994. https://doi.org/10.1210/en.2012-1044
Park, Sunghee ; Yoon, Sangyeon ; Zhao, Yuechao ; Park, Seong Eun ; Liao, Lan ; Xu, Jianming ; Lydon, John P. ; DeMayo, Francesco J. ; O'Malley, Bert W. ; Bagchi, Milan K. ; Katzenellenbogen, Benita S. / Uterine development and fertility are dependent on gene dosage of the nuclear receptor coregulator REA. In: Endocrinology. 2012 ; Vol. 153, No. 8. pp. 3982-3994.
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