Using RNA interference to study protein function.

Carol D. Curtis, Ann M. Nardulli

Research output: Contribution to journalArticlepeer-review


RNA interference can be extremely useful in determining the function of an endogenously-expressed protein in its normal cellular environment. In this chapter, we describe a method that uses small interfering RNA (siRNA) to knock down mRNA and protein expression in cultured cells so that the effect of a putative regulatory protein on gene expression can be delineated. Methods of assessing the effectiveness of the siRNA procedure using real time quantitative PCR and Western analysis are also included.

Original languageEnglish (US)
Pages (from-to)187-204
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
StatePublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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