Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays

Sherine George, Anusha Pokhriyal, Sarah I. Jones, Meng Lu, Lila O. Vodkin, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Gene expression analysis of low abundance genes remains difficult when DNA microarrays are performed on standard glass substrates. However, we have shown that by using photonic crystals (PC) made on quartz substrates, the fluorescence intensity of Cyanine-5 (Cy5) labeled microarray spots is greatly enhanced. In a 1-color microarray experiment studying gene expression of soybean cotyledon tissue, an average signal enhancement factor of 17.8 was observed on the PC. Furthermore, twice as many genes were detectable on these PCs as compared to glass. By improving the sensitivity of this fluorescent assay, low expression genes that were undetectable on glass were quantified on the PC.

Original languageEnglish (US)
Title of host publication33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
Pages26-29
Number of pages4
DOIs
StatePublished - Dec 26 2011
Event33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA, United States
Duration: Aug 30 2011Sep 3 2011

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)1557-170X

Other

Other33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
CountryUnited States
CityBoston, MA
Period8/30/119/3/11

Fingerprint

Optics and Photonics
Quartz
Microarrays
Photonic crystals
Oligonucleotide Array Sequence Analysis
Gene expression
Glass
DNA
Fluorescence
Gene Expression
Substrates
Genes
Cotyledon
Soybeans
Assays
Color
Tissue
Experiments

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

Cite this

George, S., Pokhriyal, A., Jones, S. I., Lu, M., Vodkin, L. O., & Cunningham, B. T. (2011). Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. In 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 (pp. 26-29). [6089888] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/IEMBS.2011.6089888

Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. / George, Sherine; Pokhriyal, Anusha; Jones, Sarah I.; Lu, Meng; Vodkin, Lila O.; Cunningham, Brian T.

33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. p. 26-29 6089888 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

George, S, Pokhriyal, A, Jones, SI, Lu, M, Vodkin, LO & Cunningham, BT 2011, Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. in 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011., 6089888, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, pp. 26-29, 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011, Boston, MA, United States, 8/30/11. https://doi.org/10.1109/IEMBS.2011.6089888
George S, Pokhriyal A, Jones SI, Lu M, Vodkin LO, Cunningham BT. Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. In 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. p. 26-29. 6089888. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/IEMBS.2011.6089888
George, Sherine ; Pokhriyal, Anusha ; Jones, Sarah I. ; Lu, Meng ; Vodkin, Lila O. ; Cunningham, Brian T. / Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. pp. 26-29 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).
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