Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays

Sherine George; Anusha Pokhriyal; Sarah I. Jones; Meng Lu; Lila O. Vodkin; Brian T. Cunningham

doi:10.1109/IEMBS.2011.6089888

Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays

Sherine George, Anusha Pokhriyal, Sarah I. Jones, Meng Lu, Lila O. Vodkin, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Gene expression analysis of low abundance genes remains difficult when DNA microarrays are performed on standard glass substrates. However, we have shown that by using photonic crystals (PC) made on quartz substrates, the fluorescence intensity of Cyanine-5 (Cy5) labeled microarray spots is greatly enhanced. In a 1-color microarray experiment studying gene expression of soybean cotyledon tissue, an average signal enhancement factor of 17.8 was observed on the PC. Furthermore, twice as many genes were detectable on these PCs as compared to glass. By improving the sensitivity of this fluorescent assay, low expression genes that were undetectable on glass were quantified on the PC.

Original language	English (US)
Title of host publication	33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
Pages	26-29
Number of pages	4
DOIs	https://doi.org/10.1109/IEMBS.2011.6089888
State	Published - Dec 26 2011
Event	33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 - Boston, MA, United States Duration: Aug 30 2011 → Sep 3 2011

Publication series

Name	Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
ISSN (Print)	1557-170X

Other

Other	33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011
Country	United States
City	Boston, MA
Period	8/30/11 → 9/3/11

ASJC Scopus subject areas

Signal Processing
Biomedical Engineering
Computer Vision and Pattern Recognition
Health Informatics

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George, S., Pokhriyal, A., Jones, S. I., Lu, M., Vodkin, L. O., & Cunningham, B. T. (2011). Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. In 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011 (pp. 26-29). [6089888] (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/IEMBS.2011.6089888

Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. / George, Sherine; Pokhriyal, Anusha; Jones, Sarah I.; Lu, Meng; Vodkin, Lila O.; Cunningham, Brian T.

33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. p. 26-29 6089888 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

George, S, Pokhriyal, A, Jones, SI, Lu, M, Vodkin, LO & Cunningham, BT 2011, Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. in 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011., 6089888, Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS, pp. 26-29, 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011, Boston, MA, United States, 8/30/11. https://doi.org/10.1109/IEMBS.2011.6089888

George S, Pokhriyal A, Jones SI, Lu M, Vodkin LO, Cunningham BT. Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. In 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. p. 26-29. 6089888. (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS). https://doi.org/10.1109/IEMBS.2011.6089888

George, Sherine ; Pokhriyal, Anusha ; Jones, Sarah I. ; Lu, Meng ; Vodkin, Lila O. ; Cunningham, Brian T. / Using photonic crystal enhanced fluorescence on quartz substrates to improve the sensitivity of DNA microarrays. 33rd Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS 2011. 2011. pp. 26-29 (Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS).

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