Using microfluidics to control the extracellular environment and to measure release from selected neurons

Jonathan V Sweedler; Ming Zhong; Jennifer N. Hanson; Kyubong Jo; Larry Millet; Stanislav Rubakhin; Martha L Gillette; Ralph G Nuzzo

Using microfluidics to control the extracellular environment and to measure release from selected neurons

Jonathan V Sweedler, Ming Zhong, Jennifer N. Hanson, Kyubong Jo, Larry Millet, Stanislav Rubakhin, Martha L Gillette, Ralph G Nuzzo

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Understanding function in healthy and diseased brains requires knowledge of the biochemistry occurring within the neurons and supporting cells making up the brain. What is the cell-to-cell variation of the neurotransmitters and neuromodulators in the nervous system and how does this change in an activity-dependent manner? For many compounds, the answer is unknown. Here we use a range of unique sampling protocols, capillary-scale separations, and microfluidic devices with both freshly isolated and cultured primary neurons to probe neurotransmitter and neuropeptide content and release from neurons in well-defined networks and from well-controlled microenvironments.

Original language	English (US)
Title of host publication	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
Publisher	Chemical and Biological Microsystems Society
Pages	3-5
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: Oct 12 2008 → Oct 16 2008

Other

Other	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	10/12/08 → 10/16/08

Keywords

Cell culture
Cellular release
Mass spectrometry
Microfluidics
Neurons
Single cell

ASJC Scopus subject areas

Chemical Engineering (miscellaneous)
Bioengineering

Library availability

Discover UIUC Full Text

Cite this

Sweedler, J. V., Zhong, M., Hanson, J. N., Jo, K., Millet, L., Rubakhin, S., Gillette, M. L., & Nuzzo, R. G. (2008). Using microfluidics to control the extracellular environment and to measure release from selected neurons. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 3-5). Chemical and Biological Microsystems Society.

Using microfluidics to control the extracellular environment and to measure release from selected neurons. / Sweedler, Jonathan V; Zhong, Ming; Hanson, Jennifer N. et al.
12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, 2008. p. 3-5.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Sweedler, JV, Zhong, M, Hanson, JN, Jo, K, Millet, L, Rubakhin, S , Gillette, ML & Nuzzo, RG 2008, Using microfluidics to control the extracellular environment and to measure release from selected neurons. in 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference. Chemical and Biological Microsystems Society, pp. 3-5, 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States, 10/12/08.

@inproceedings{fcc18a43efb64191b5203ef955a2d517,

title = "Using microfluidics to control the extracellular environment and to measure release from selected neurons",

abstract = "Understanding function in healthy and diseased brains requires knowledge of the biochemistry occurring within the neurons and supporting cells making up the brain. What is the cell-to-cell variation of the neurotransmitters and neuromodulators in the nervous system and how does this change in an activity-dependent manner? For many compounds, the answer is unknown. Here we use a range of unique sampling protocols, capillary-scale separations, and microfluidic devices with both freshly isolated and cultured primary neurons to probe neurotransmitter and neuropeptide content and release from neurons in well-defined networks and from well-controlled microenvironments.",

keywords = "Cell culture, Cellular release, Mass spectrometry, Microfluidics, Neurons, Single cell",

author = "Sweedler, {Jonathan V} and Ming Zhong and Hanson, {Jennifer N.} and Kyubong Jo and Larry Millet and Stanislav Rubakhin and Gillette, {Martha L} and Nuzzo, {Ralph G}",

year = "2008",

language = "English (US)",

pages = "3--5",

booktitle = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference",

publisher = "Chemical and Biological Microsystems Society",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

TY - GEN

T1 - Using microfluidics to control the extracellular environment and to measure release from selected neurons

AU - Sweedler, Jonathan V

AU - Zhong, Ming

AU - Hanson, Jennifer N.

AU - Jo, Kyubong

AU - Millet, Larry

AU - Rubakhin, Stanislav

AU - Gillette, Martha L

AU - Nuzzo, Ralph G

PY - 2008

Y1 - 2008

N2 - Understanding function in healthy and diseased brains requires knowledge of the biochemistry occurring within the neurons and supporting cells making up the brain. What is the cell-to-cell variation of the neurotransmitters and neuromodulators in the nervous system and how does this change in an activity-dependent manner? For many compounds, the answer is unknown. Here we use a range of unique sampling protocols, capillary-scale separations, and microfluidic devices with both freshly isolated and cultured primary neurons to probe neurotransmitter and neuropeptide content and release from neurons in well-defined networks and from well-controlled microenvironments.

AB - Understanding function in healthy and diseased brains requires knowledge of the biochemistry occurring within the neurons and supporting cells making up the brain. What is the cell-to-cell variation of the neurotransmitters and neuromodulators in the nervous system and how does this change in an activity-dependent manner? For many compounds, the answer is unknown. Here we use a range of unique sampling protocols, capillary-scale separations, and microfluidic devices with both freshly isolated and cultured primary neurons to probe neurotransmitter and neuropeptide content and release from neurons in well-defined networks and from well-controlled microenvironments.

KW - Cell culture

KW - Cellular release

KW - Mass spectrometry

KW - Microfluidics

KW - Neurons

KW - Single cell

UR - http://www.scopus.com/inward/record.url?scp=84902522961&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84902522961&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84902522961

SP - 3

EP - 5

BT - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference

PB - Chemical and Biological Microsystems Society

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

Using microfluidics to control the extracellular environment and to measure release from selected neurons

Abstract

Other

Keywords

ASJC Scopus subject areas

Library availability

Related links

Fingerprint

Cite this