Abstract

Understanding function in healthy and diseased brains requires knowledge of the biochemistry occurring within the neurons and supporting cells making up the brain. What is the cell-to-cell variation of the neurotransmitters and neuromodulators in the nervous system and how does this change in an activity-dependent manner? For many compounds, the answer is unknown. Here we use a range of unique sampling protocols, capillary-scale separations, and microfluidic devices with both freshly isolated and cultured primary neurons to probe neurotransmitter and neuropeptide content and release from neurons in well-defined networks and from well-controlled microenvironments.

Original languageEnglish (US)
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages3-5
Number of pages3
StatePublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Cell culture
  • Cellular release
  • Mass spectrometry
  • Microfluidics
  • Neurons
  • Single cell

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

Fingerprint Dive into the research topics of 'Using microfluidics to control the extracellular environment and to measure release from selected neurons'. Together they form a unique fingerprint.

  • Cite this

    Sweedler, J. V., Zhong, M., Hanson, J. N., Jo, K., Millet, L., Rubakhin, S., Gillette, M. L., & Nuzzo, R. G. (2008). Using microfluidics to control the extracellular environment and to measure release from selected neurons. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 3-5). Chemical and Biological Microsystems Society.