Using microfluidics and mass spectrometry to study peptide release in neurons

C. A. Croushore, C. Y. Lee, M. Zhong, Jonathan V Sweedler

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present here two microfluidic devices designed to create and maintain a neuronal network and characterize its response to chemical stimulation using mass spectrometry. The first microfluidic device is composed of microvalve controlled PDMS channels that are adhered to an octadecyltrichlorosilane functionalized silicon wafer. This surface functionalization provides for neuropeptide releasate collection through hydrophobic interactions. Following collection, the adsorbed peptides are interrogated on-chip using mass spectrometry imaging. A second microfluidic device consists of a main channel with two stimulation channels that provides for off-line collection and detection of peptide releasates with precise control over chemical stimulations applied to cultured neurons.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages858-860
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Mass spectrometry
  • Microfluidics
  • Microvalves
  • Neurons

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Croushore, C. A., Lee, C. Y., Zhong, M., & Sweedler, J. V. (2011). Using microfluidics and mass spectrometry to study peptide release in neurons. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 858-860). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).