Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles

Joseph T. Buchman, Ali Rahnamoun, Kaitlin M. Landy, Xi Zhang, Ariane M. Vartanian, Lisa M. Jacob, Catherine J. Murphy, Rigoberto Hernandez, Christy L. Haynes

Research output: Contribution to journalArticle

Abstract

We aim to establish the effect of environmental diversity in evaluating nanotoxicity to bacteria. We assessed the toxicity of 4 nm polyallylamine hydrochloride-wrapped gold nanoparticles (PAH AuNPs) to a panel of bacteria from diverse environmental niches. The bacteria experienced a range of toxicities as evidenced by the different minimum bactericidal concentrations determined; the sensitivities of the bacteria was A. vinelandii = P. aeruginosa > S. oneidensis MR-4 > A. baylyi > S. oneidensis MR-1. Interactions between gold nanoparticles and molecular components of the cell wall were investigated by TEM, flow cytometry, and computational modeling. Binding results showed a general trend that bacteria with smooth lipopolysaccharides (LPS) bind more PAH AuNPs than bacteria with rough LPS. Computational models reveal that PAH migrates to phosphate groups in the core of the LPS structure. Overall, our results demonstrate that simple interactions between nanoparticles and the bacterial cell wall cannot fully account for observed trends in toxicity, which points to the importance of establishing more comprehensive approaches for modeling environmental nanotoxicity.

Original languageEnglish (US)
Pages (from-to)279-288
Number of pages10
JournalEnvironmental Science: Nano
Volume5
Issue number2
DOIs
StatePublished - Jan 1 2018

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Gold
Toxicity
Bacteria
gold
Nanoparticles
toxicity
bacterium
Polycyclic aromatic hydrocarbons
Lipopolysaccharides
PAH
Cells
phosphate group
environmental modeling
Flow cytometry
flow cytometry
polyallylamine
nanoparticle
transmission electron microscopy
niche
Phosphates

ASJC Scopus subject areas

  • Materials Science (miscellaneous)
  • Environmental Science(all)

Cite this

Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles. / Buchman, Joseph T.; Rahnamoun, Ali; Landy, Kaitlin M.; Zhang, Xi; Vartanian, Ariane M.; Jacob, Lisa M.; Murphy, Catherine J.; Hernandez, Rigoberto; Haynes, Christy L.

In: Environmental Science: Nano, Vol. 5, No. 2, 01.01.2018, p. 279-288.

Research output: Contribution to journalArticle

Buchman, Joseph T. ; Rahnamoun, Ali ; Landy, Kaitlin M. ; Zhang, Xi ; Vartanian, Ariane M. ; Jacob, Lisa M. ; Murphy, Catherine J. ; Hernandez, Rigoberto ; Haynes, Christy L. / Using an environmentally-relevant panel of Gram-negative bacteria to assess the toxicity of polyallylamine hydrochloride-wrapped gold nanoparticles. In: Environmental Science: Nano. 2018 ; Vol. 5, No. 2. pp. 279-288.
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