Use of transcriptome data to unravel the fine structure of genes involved in sepsis

Brian J. Stevenson, Christian Iseli, Bruce Beutler, C. Victor Jongeneel

Research output: Contribution to journalArticlepeer-review


The sequence of the human genome is providing researchers with the scaffold upon which genes are built. The definition of the boundaries of the genes themselves and of their complex architecture requires a mapping of the transcriptome to the genome. A methodology was developed for generating a detailed transcriptome map and for reconstituting transcripts by using the genome as a template. As a demonstration of the potential of this method, the structure of the human Toll-like receptor (TLR) genes was reevaluated. For all TLR genes for which a genomic sequence was available (i.e., all except TLR10), novel features of the gene structure were discovered. These features include multiple alternative polyadenylation sites, additional exons or splice variants, and overlaps with other genes. These findings have implications for the analysis of TLR gene expression and for the diversity of the proteins encoded by these genes.

Original languageEnglish (US)
Pages (from-to)S308-S314
JournalJournal of Infectious Diseases
Issue numberSUPPL. 2
StatePublished - Jun 15 2003
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases


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