Use of thiotriphosphates for the study of in vitro initiation in adenovirus-infected cell nuclei

David Bunick; Roberto Weinmann

doi:10.1016/0005-2787(80)90014-3

Use of thiotriphosphates for the study of in vitro initiation in adenovirus-infected cell nuclei

David Bunick
, Roberto Weinmann

Research output: Contribution to journal › Article › peer-review

Abstract

The use of thiotriphosphates as a means of analyzing in vitro initiation products of adenovirus-infected cells is demonstrated for the RNA polymerase III transcript VA-RNA. Results suggest its usefulness in discriminating between adenosine- or guanosine-initiated transcripts and in selecting the 5′-oligonucleotide.

Original language	English (US)
Pages (from-to)	331-338
Number of pages	8
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	610
Issue number	2
DOIs	https://doi.org/10.1016/0005-2787(80)90014-3
State	Published - Dec 11 1980
Externally published	Yes

Keywords

Initiation
Thiotriphosphate
Transcription
Virus infection

ASJC Scopus subject areas

General Medicine

Online availability

10.1016/0005-2787(80)90014-3

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abstract = "The use of thiotriphosphates as a means of analyzing in vitro initiation products of adenovirus-infected cells is demonstrated for the RNA polymerase III transcript VA-RNA. Results suggest its usefulness in discriminating between adenosine- or guanosine-initiated transcripts and in selecting the 5′-oligonucleotide.",

keywords = "Initiation, Thiotriphosphate, Transcription, Virus infection",

author = "David Bunick and Roberto Weinmann",

note = "We would like to thank T. Shenk for making results available before publication. A. Naylor and B. Crutchfield provided invaluable technical assistance. This work was performed with support from the National Institutes of Health, grants AI-13231 and CA-21124 to R.W. and Training Grant PHS 5T32 07229-05 to D.B.",

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doi = "10.1016/0005-2787(80)90014-3",

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AU - Bunick, David

AU - Weinmann, Roberto

N1 - We would like to thank T. Shenk for making results available before publication. A. Naylor and B. Crutchfield provided invaluable technical assistance. This work was performed with support from the National Institutes of Health, grants AI-13231 and CA-21124 to R.W. and Training Grant PHS 5T32 07229-05 to D.B.

PY - 1980/12/11

Y1 - 1980/12/11

N2 - The use of thiotriphosphates as a means of analyzing in vitro initiation products of adenovirus-infected cells is demonstrated for the RNA polymerase III transcript VA-RNA. Results suggest its usefulness in discriminating between adenosine- or guanosine-initiated transcripts and in selecting the 5′-oligonucleotide.

AB - The use of thiotriphosphates as a means of analyzing in vitro initiation products of adenovirus-infected cells is demonstrated for the RNA polymerase III transcript VA-RNA. Results suggest its usefulness in discriminating between adenosine- or guanosine-initiated transcripts and in selecting the 5′-oligonucleotide.

KW - Initiation

KW - Thiotriphosphate

KW - Transcription

KW - Virus infection

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UR - https://www.scopus.com/pages/publications/0019161588#tab=citedBy

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DO - 10.1016/0005-2787(80)90014-3

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JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 2

ER -

Use of thiotriphosphates for the study of in vitro initiation in adenovirus-infected cell nuclei

Abstract

Keywords

ASJC Scopus subject areas

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