Use of the progesterone receptor antagonist RU 486 to identify novel progesterone receptor-regulated pathways in implantation

Indrani C. Bagchi, Quanxi Li, Yong Pil Cheon, Srinivasa Raju Mantena, Athilakshmi Kannan, Milan K. Bagchi

Research output: Contribution to journalReview article

Abstract

The steroid hormone progesterone (P) is a critical regulator of embryo implantation and maintenance of pregnancy. P acting through the nuclear progesterone receptors (PRs) regulates the expression of specific gene networks that in turn control the extensive cell proliferation, differentiation, and remodeling that occur in various uterine cell types during the progressive phases of implantation. To identify the P-regulated pathways that underlie the implantation process in the mouse, we employed RU 486, a well-characterized PR antagonist that binds to the receptor and blocks its gene regulatory function. We performed messenger RNA (mRNA) profiling in the peri-implantation uterus using oligonucleotide microarrays to analyze changes in mRNA levels in response to RU 486. This analysis provided, for the first time, a comprehensive profile of PR-regulated gene networks with potential roles during implantation. Our study identified a variety of novel PR-regulated molecules, such as growth factors, protease inhibitors, metabolic enzymes, peptide hormones, transcription factors, immune response molecules, cytoskeletal proteins, and cell adhesion molecules, that are potential mediators of P action in the periimplantation mouse uterus. This article provides a brief description of the expression and function of these newly identified molecular pathways.

Original languageEnglish (US)
Pages (from-to)38-45
Number of pages8
JournalSeminars in Reproductive Medicine
Volume23
Issue number1
DOIs
StatePublished - Feb 1 2005

Fingerprint

Mifepristone
Progesterone Receptors
Gene Regulatory Networks
Uterus
Pregnancy Maintenance
Messenger RNA
Cytoskeletal Proteins
Peptide Hormones
Cell Adhesion Molecules
Regulator Genes
Microarray Analysis
Cytoplasmic and Nuclear Receptors
Protease Inhibitors
Oligonucleotide Array Sequence Analysis
Progesterone
Cell Differentiation
Intercellular Signaling Peptides and Proteins
Transcription Factors
Steroids
Cell Proliferation

Keywords

  • Antiprogestin RU 486
  • Implantation
  • Progesterone
  • Receptor

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Reproductive Medicine
  • Endocrinology
  • Obstetrics and Gynecology
  • Physiology (medical)

Cite this

Use of the progesterone receptor antagonist RU 486 to identify novel progesterone receptor-regulated pathways in implantation. / Bagchi, Indrani C.; Li, Quanxi; Cheon, Yong Pil; Mantena, Srinivasa Raju; Kannan, Athilakshmi; Bagchi, Milan K.

In: Seminars in Reproductive Medicine, Vol. 23, No. 1, 01.02.2005, p. 38-45.

Research output: Contribution to journalReview article

@article{af8e7144591a423bbecf62123df0ee95,
title = "Use of the progesterone receptor antagonist RU 486 to identify novel progesterone receptor-regulated pathways in implantation",
abstract = "The steroid hormone progesterone (P) is a critical regulator of embryo implantation and maintenance of pregnancy. P acting through the nuclear progesterone receptors (PRs) regulates the expression of specific gene networks that in turn control the extensive cell proliferation, differentiation, and remodeling that occur in various uterine cell types during the progressive phases of implantation. To identify the P-regulated pathways that underlie the implantation process in the mouse, we employed RU 486, a well-characterized PR antagonist that binds to the receptor and blocks its gene regulatory function. We performed messenger RNA (mRNA) profiling in the peri-implantation uterus using oligonucleotide microarrays to analyze changes in mRNA levels in response to RU 486. This analysis provided, for the first time, a comprehensive profile of PR-regulated gene networks with potential roles during implantation. Our study identified a variety of novel PR-regulated molecules, such as growth factors, protease inhibitors, metabolic enzymes, peptide hormones, transcription factors, immune response molecules, cytoskeletal proteins, and cell adhesion molecules, that are potential mediators of P action in the periimplantation mouse uterus. This article provides a brief description of the expression and function of these newly identified molecular pathways.",
keywords = "Antiprogestin RU 486, Implantation, Progesterone, Receptor",
author = "Bagchi, {Indrani C.} and Quanxi Li and Cheon, {Yong Pil} and Mantena, {Srinivasa Raju} and Athilakshmi Kannan and Bagchi, {Milan K.}",
year = "2005",
month = "2",
day = "1",
doi = "10.1055/s-2005-864032",
language = "English (US)",
volume = "23",
pages = "38--45",
journal = "Seminars in Reproductive Medicine",
issn = "1526-8004",
publisher = "Thieme Medical Publishers",
number = "1",

}

TY - JOUR

T1 - Use of the progesterone receptor antagonist RU 486 to identify novel progesterone receptor-regulated pathways in implantation

AU - Bagchi, Indrani C.

AU - Li, Quanxi

AU - Cheon, Yong Pil

AU - Mantena, Srinivasa Raju

AU - Kannan, Athilakshmi

AU - Bagchi, Milan K.

PY - 2005/2/1

Y1 - 2005/2/1

N2 - The steroid hormone progesterone (P) is a critical regulator of embryo implantation and maintenance of pregnancy. P acting through the nuclear progesterone receptors (PRs) regulates the expression of specific gene networks that in turn control the extensive cell proliferation, differentiation, and remodeling that occur in various uterine cell types during the progressive phases of implantation. To identify the P-regulated pathways that underlie the implantation process in the mouse, we employed RU 486, a well-characterized PR antagonist that binds to the receptor and blocks its gene regulatory function. We performed messenger RNA (mRNA) profiling in the peri-implantation uterus using oligonucleotide microarrays to analyze changes in mRNA levels in response to RU 486. This analysis provided, for the first time, a comprehensive profile of PR-regulated gene networks with potential roles during implantation. Our study identified a variety of novel PR-regulated molecules, such as growth factors, protease inhibitors, metabolic enzymes, peptide hormones, transcription factors, immune response molecules, cytoskeletal proteins, and cell adhesion molecules, that are potential mediators of P action in the periimplantation mouse uterus. This article provides a brief description of the expression and function of these newly identified molecular pathways.

AB - The steroid hormone progesterone (P) is a critical regulator of embryo implantation and maintenance of pregnancy. P acting through the nuclear progesterone receptors (PRs) regulates the expression of specific gene networks that in turn control the extensive cell proliferation, differentiation, and remodeling that occur in various uterine cell types during the progressive phases of implantation. To identify the P-regulated pathways that underlie the implantation process in the mouse, we employed RU 486, a well-characterized PR antagonist that binds to the receptor and blocks its gene regulatory function. We performed messenger RNA (mRNA) profiling in the peri-implantation uterus using oligonucleotide microarrays to analyze changes in mRNA levels in response to RU 486. This analysis provided, for the first time, a comprehensive profile of PR-regulated gene networks with potential roles during implantation. Our study identified a variety of novel PR-regulated molecules, such as growth factors, protease inhibitors, metabolic enzymes, peptide hormones, transcription factors, immune response molecules, cytoskeletal proteins, and cell adhesion molecules, that are potential mediators of P action in the periimplantation mouse uterus. This article provides a brief description of the expression and function of these newly identified molecular pathways.

KW - Antiprogestin RU 486

KW - Implantation

KW - Progesterone

KW - Receptor

UR - http://www.scopus.com/inward/record.url?scp=14744301710&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14744301710&partnerID=8YFLogxK

U2 - 10.1055/s-2005-864032

DO - 10.1055/s-2005-864032

M3 - Review article

C2 - 15714388

AN - SCOPUS:14744301710

VL - 23

SP - 38

EP - 45

JO - Seminars in Reproductive Medicine

JF - Seminars in Reproductive Medicine

SN - 1526-8004

IS - 1

ER -