Abstract
Milk growth factors are thought to contribute to postnatal gastrointestinal growth. The roles of epidermal growth factor (EGF) and insulin-like growth factor-I (IGF-I) in the neonatal piglet intestine were investigated. In the first study, piglets were infected with rotavirus on d 4 postpartum and received formula containing 0, 500 or 1000 μg/l of EGF for 8 days. A non-infected control group received no EGF. Infected piglets developed severe diarrhea and gained 60% less weight than controls. Rotaviral infection caused a 37% decrease in villus height and 40% decreases in intestinal enzyme activities compared to control. Oral EGF increased villus height and lactase activity in a linear dose-response fashion. Our results suggest that supplementation of formulas with high physiological levels of EGF may aid in small intestinal recovery. A second study investigated absorption of orally administered IGF-I. Newborn piglets were fitted with catheters via the umbilical artery and vein. Piglets were given formula containing 25 μCi of [125I]-IGF-I and blood samples were drawn for 240 min. Total radioactivity, protein bound counts, and counts immunoprecipitable with an antibody to IGF-I were determined in plasma. Radioactivity was detected in portal and arterial plasma within 15 min and rose throughout the study, however, protein bound counts were stable at 20-30% of total counts between 30 and 180 min post-gavage. Approximately 10% of the counts were immunoprecipitable by a polyclonal antibody to IGF-I, suggesting that up to 10% of orally administered IGF-I may be absorbed intact.
Original language | English (US) |
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Pages (from-to) | 153-162 |
Number of pages | 10 |
Journal | Endocrine Regulations |
Volume | 28 |
Issue number | 4 |
State | Published - 1994 |
Keywords
- IGF binding proteins (IGFBPs)
- epidermal growth factor (EGF)
- insulin-like growth factor (IGF)
- intestine
- milk
- piglet
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Endocrinology