Use of recombinant S1 spike polypeptide to develop a TCoV-specific antibody ELISA

Maged H. Gomaa, Dongwan Yoo, Davor Ojkic, John R. Barta

Research output: Contribution to journalArticle

Abstract

Turkey coronavirus (TCoV) causes diarrhoea in young turkey poults but little is known about its prevalence in the field. To address this, a portion of the S1 region of the spike glycoprotein of TCoV carrying relevant B cell epitopes (amino acid positions 54-395) was cloned and expressed in Escherichia coli. This protein was purified and used to develop an indirect ELISA for detection of antibodies against TCoV. Using experimentally derived positive and negative turkey serum samples this ELISA showed high sensitivity (95%) and specificity (92%) for TCoV. To further evaluate the potential of the ELISA, 360 serum samples from commercial turkey farms in Ontario were tested for TCoV-specific antibodies using the recombinant TCoV ELISA. High seroprevalence of TCoV was found with 71.11% of breeders and 56.67% of meat turkeys testing seropositive. Although there was significant positive correlation with a TCoV-N protein-based ELISA, there was little to no correlation with the whole IBV antigen-based ELISA when field sera were tested for antibodies against TCoV.

Original languageEnglish (US)
Pages (from-to)281-288
Number of pages8
JournalVeterinary Microbiology
Volume138
Issue number3-4
DOIs
StatePublished - Sep 18 2009

Keywords

  • Antibody
  • Enzyme immunoassay
  • IBV
  • Seroprevalence
  • Spike protein
  • TCoV

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

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