Use of recombinant human thyroid-stimulating hormone for thyrotropin stimulation testing in euthyroid ferrets

Jörg Mayer, Robert Wagner, Mark A. Mitchell, Kellie Fecteau

Research output: Contribution to journalArticlepeer-review

Abstract

Objective-To evaluate the effects of IM administration of recombinant human thyroid-stimulating hormone (rhTSH) on plasma total thyroxine (T4) concentrations in euthyroid ferrets. Design-Evaluation study. Animals-25 healthy neutered ferrets (14 female and 11 male) of various ages from 2 populations (laboratory ferrets from Georgia and pet ferrets from Pennsylvania). Procedures-Each ferret underwent a physical examination and standard hematologic testing to ensure it was healthy and had clinically normal thyroid function. Once determined to be euthyroid, ferrets received a single IM injection of 100 μg of rhTSH. Blood samples were collected into plasma-separator tubes immediately before the rhTSH injection (time 0) and 4 hours after injection to measure T4 concentrations. Results-Males did not differ from females in regard to prestimulation or poststimulation plasma T4 concentrations; however, prestimulation and poststimulation T4 concentrations were significantly different between the 2 groups of ferrets. A significant difference was also identified between prestimulation T4 concentration (mean ± SD, 21.3 ± 6.1 nmol/L) and poststimulation T4 concentration (29.9 ± 8.2 nmol/L). All 25 ferrets had high poststimulation T4 concentrations (median difference, 7.5 nmol/L; 10% to 90% interval, 3.26 to 17.70 nmol/L [0.25 to 1.38 μg/dL]; range, 2.50 to 20.70 nmol/L [0.19 to 1.61 μg/dL]); this represented a median increase in T4 concentration of 35% (10% to 90% interval, 18% to 81%; range, 8% to 126%). Conclusions and Clinical Relevance-Results suggested that rhTSH can be used for thyrotropin stimulation testing in ferrets when administered IM. According to the findings, a euthyroid ferret should have an increase of approximately 30% in plasma T4 concentration 4 hours after rhTSH injection.

Original languageEnglish (US)
Pages (from-to)1432-1435
Number of pages4
JournalJournal of the American Veterinary Medical Association
Volume243
Issue number10
DOIs
StatePublished - Nov 15 2013

ASJC Scopus subject areas

  • veterinary(all)

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