Use of random amplified polymorphic DNA (RAPD) for identification of Gaeumannomyces species

Hanafy M. Fouly, Henry T. Wilkinson, Leslie L. Domier

Research output: Contribution to journalArticle

Abstract

Randomly amplified polymorphic DNAs (RAPD) were used to distinguish among isolates of Gaeumannomyces graminis var. tritici, G. g. var. graminis, G. g. var. avenue, G. incrustans, and G. cylindrosporous. Of 60 random sequence decamer primers tested, 28 produced bands and 15 showed evidence of polymorphism. Four primers produced DNA amplification patterns that were used to distinguish G. graminis, G. incrustans, and G. cylindrosporous. Banding patterns were similar within isolates of G. g. var. tritici, G. g. var. avenae and G. incrustans. G. g. var. graminis showed greater variability in banding patterns. The unweighted pair group method with arithmetical averages (UPGMA) indicated that G. g. var. tritici isolates were more closely related to G. g. var. avenae than to G. g. var. graminis isolates. The results of our study showed thai RAPD markers can be used to confirm the identification of Gaeumannomyces species and varieties.

Original languageEnglish (US)
Pages (from-to)703-710
Number of pages8
JournalSoil Biology and Biochemistry
Volume28
Issue number6
DOIs
StatePublished - Jun 1996

ASJC Scopus subject areas

  • Microbiology
  • Soil Science

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