Optical coherence tomography (OCT) images of biological tissues often have low contrast Spectroscopic OCT (SOCT) methods have been developed to enhance contrast. Due to the time-frequency "uncertainty principle" in SOCT signal analysis, minute spectral changes can not be detected for high spatial resolution applications. In order to enhance SOCT contrast in tissues with low inherent spectral absorption, we developed a contrast enhancing technique using near-infrared (NIR) dyes. We developed an optimized algorithm for SOCT and an optimum dye concentration for imaging. The effect of contrast enhancement was studied both in tissue phantoms and in situ experiments. Contrast-enhanced images are compared with fluorescence microscopy, demonstrating a link between SOCT and fluorescence imaging.

Original languageEnglish (US)
Pages (from-to)1214-1217
Number of pages4
JournalAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Volume26 II
StatePublished - 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: Sep 1 2004Sep 5 2004


  • Fluorescence
  • Near infrared dye
  • Optical coherence tomography
  • Spectroscopic

ASJC Scopus subject areas

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics


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