Use of molecular beacons to study mixing and hybridization in microfluidic devices

Chuanwu Xi, M. Balberg, J. Selby, S. Boppart, L. Raskin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We present the use of molecular beacons as sensitive indicators in microfluidic bioMEM devices. A method has been developed to study mixing and hybridization of molecular beacons to target nucleic acids in real time in a microfluidic channel. This method allows quantification of DNA and RNA molecules in solution, i.e., without the prerequisite of an immobilization step necessary in most other hybridization formats. The detection limit for DNA was determined to be in the order of femtomoles. Our results suggest the feasibility of using molecular beacon hybridization in microfluidic devices for the detection of pathogenic bacteria in environmental and medical fluids.

Original languageEnglish (US)
Title of host publication2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings
EditorsDavid Beebe, Andre Dittmar
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages541-544
Number of pages4
ISBN (Electronic)0780374800, 9780780374805
DOIs
StatePublished - Jan 1 2002
Event2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Madison, United States
Duration: May 2 2002May 4 2002

Publication series

Name2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings

Other

Other2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology
CountryUnited States
CityMadison
Period5/2/025/4/02

Fingerprint

Lab-On-A-Chip Devices
Microfluidics
DNA
Immobilization
Nucleic Acids
Limit of Detection
RNA
Bacteria

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology (medical)

Cite this

Xi, C., Balberg, M., Selby, J., Boppart, S., & Raskin, L. (2002). Use of molecular beacons to study mixing and hybridization in microfluidic devices. In D. Beebe, & A. Dittmar (Eds.), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings (pp. 541-544). [1002404] (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MMB.2002.1002404

Use of molecular beacons to study mixing and hybridization in microfluidic devices. / Xi, Chuanwu; Balberg, M.; Selby, J.; Boppart, S.; Raskin, L.

2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. ed. / David Beebe; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. p. 541-544 1002404 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xi, C, Balberg, M, Selby, J, Boppart, S & Raskin, L 2002, Use of molecular beacons to study mixing and hybridization in microfluidic devices. in D Beebe & A Dittmar (eds), 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings., 1002404, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings, Institute of Electrical and Electronics Engineers Inc., pp. 541-544, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology, Madison, United States, 5/2/02. https://doi.org/10.1109/MMB.2002.1002404
Xi C, Balberg M, Selby J, Boppart S, Raskin L. Use of molecular beacons to study mixing and hybridization in microfluidic devices. In Beebe D, Dittmar A, editors, 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. Institute of Electrical and Electronics Engineers Inc. 2002. p. 541-544. 1002404. (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings). https://doi.org/10.1109/MMB.2002.1002404
Xi, Chuanwu ; Balberg, M. ; Selby, J. ; Boppart, S. ; Raskin, L. / Use of molecular beacons to study mixing and hybridization in microfluidic devices. 2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings. editor / David Beebe ; Andre Dittmar. Institute of Electrical and Electronics Engineers Inc., 2002. pp. 541-544 (2nd Annual International IEEE-EMBS Special Topic Conference on Microtechnologies in Medicine and Biology - Proceedings).
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