Abstract

We present the use of DNA and peptide nucleic acid (PNA) molecular beacons (MBs) as sensitive indicators in microfluidic bioMEM devices. DNA and PNA MBs can be used to quantitatively study hybridization kinetics in real time in a polydimethyl siloxane (PDMS) microfluidic device. PNA MBs perform better than DNA MBs for the study of hybridization kinetics of rRNA targets in real time in microfluidic channels. We also demonstrate the use of PNA MBs for fast detection of bacterial cells in microfluidic channels. Using PNA MBs as detection probes will enable us to develop an integrated biosensor for the rapid and on-site detection and quantification of microbial pathogens in environmental and clinical samples.

Original languageEnglish (US)
Pages (from-to)170-177
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4982
DOIs
StatePublished - May 26 2003
EventMicrofluidics, BioMEMS, and Medical Microsystems - San Jose, CA, United States
Duration: Jan 27 2003Jan 29 2003

Fingerprint

Peptide Nucleic Acids
beacons
microfluidic devices
Microfluidics
Peptides
Bacteria
bacteria
nucleic acids
peptides
DNA
deoxyribonucleic acid
Kinetics
Siloxanes
Biosensor
Pathogens
pathogens
Biosensors
siloxanes
Quantification
kinetics

Keywords

  • Biosensor
  • Hybridization
  • Molecular beacon
  • Peptide nucleic acid (PNA)

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Use of molecular beacons for the detection of bacteria in microfluidic devices. / Xi, Chuanwu; Boppart, Stephen; Raskin, Lutgarde.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 4982, 26.05.2003, p. 170-177.

Research output: Contribution to journalConference article

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AB - We present the use of DNA and peptide nucleic acid (PNA) molecular beacons (MBs) as sensitive indicators in microfluidic bioMEM devices. DNA and PNA MBs can be used to quantitatively study hybridization kinetics in real time in a polydimethyl siloxane (PDMS) microfluidic device. PNA MBs perform better than DNA MBs for the study of hybridization kinetics of rRNA targets in real time in microfluidic channels. We also demonstrate the use of PNA MBs for fast detection of bacterial cells in microfluidic channels. Using PNA MBs as detection probes will enable us to develop an integrated biosensor for the rapid and on-site detection and quantification of microbial pathogens in environmental and clinical samples.

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